| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIMM21 |
| Uniprot No | Q9BVV7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-248aa |
| 氨基酸序列 | MICTFLRAVQYTEKLHRSSAKRLLLPYIVLNKACLKTEPSLRCGLQYQKKTLRPRCILGVTQKTIWTQGPSPRKAKEDGSKQVSVHRSQRGGTAVPTSQKVKEAGRDFTYLIVVLFGISITGGLFYTIFKELFSSSSPSKIYGRALEKCRSHPEVIGVFGESVKGYGEVTRRGRRQHVRFTEYVKDGLKHTCVKFYIEGSEPGKQGTVYAQVKENPGSGEYDFRYIFVEIESYPRRTIIIEDNRSQDD |
| 预测分子量 | 55.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TIMM21重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *Role of Tim21 in mitochondrial trafficking and protein import*
**作者**: Chacinska, A., et al.
**摘要**: 本研究解析了TIMM21作为TIM23复合体亚基的功能，通过重组蛋白表达实验证实其参与前体蛋白的线粒体靶向转运。实验表明，TIMM21重组蛋白可增强TIM23复合体与内膜电位的耦合，调控前体蛋白的膜电位依赖性运输。
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2. **文献名称**: *Reconstitution of the human TIM23 complex reveals critical roles of Tim21 in membrane potential sensing*
**作者**: Mokranjac, D., et al.
**摘要**: 作者利用重组TIMM21蛋白与TIM23复合体其他组分进行体外重构，证明TIMM21通过结合内膜电势激活转运通道，并调控前体蛋白的输入效率。重组蛋白实验揭示了其结构域对复合体组装的关键作用。
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3. **文献名称**: *Purification and functional analysis of recombinant human TIMM21 for mitochondrial import studies*
**作者**: Kang, Y., et al.
**摘要**: 本研究报道了人源TIMM21重组蛋白在大肠杆菌中的高效表达与纯化方案，并利用体外线粒体导入实验验证其功能。结果表明，重组TIMM21能恢复Tim21缺陷型线粒体的蛋白输入缺陷，证实其分子伴侣活性。
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*注：以上文献信息为示例性质，实际研究中建议通过PubMed或Web of Science以“TIMM21 recombinant”或“TIMM21 purification”为关键词检索最新文献。*
**Background of TIMM21 Recombinant Protein**
TIMM21 (Translocase of Inner Mitochondrial Membrane 21) is a key component of the mitochondrial TIM23 complex, which mediates the import of nuclear-encoded precursor proteins into the mitochondrial matrix. This process is critical for maintaining mitochondrial function, as the majority of mitochondrial proteins are synthesized in the cytosol and require precise targeting and translocation. TIMM21 specifically acts as a receptor, recognizing precursor proteins with N-terminal presequences and facilitating their docking to the TIM23 translocase. It also plays a role in coupling protein translocation to the mitochondrial membrane potential, ensuring efficient import.
Recombinant TIMM21 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional TIMM21 for *in vitro* studies. Its recombinant form allows researchers to investigate the molecular mechanisms of mitochondrial protein import, including interactions with chaperones, translocase subunits, and precursor substrates. Structural studies using recombinant TIMM21 have provided insights into its domain organization, particularly its N-terminal presequence-binding region and C-terminal membrane-anchoring domain.
Dysregulation of TIMM21 is linked to mitochondrial disorders, neurodegeneration, and cancer, making it a potential therapeutic target. Recombinant TIMM21 enables drug screening for compounds that modulate protein import efficiency or restore mitochondrial homeostasis. Additionally, it serves as a tool to study diseases associated with mitochondrial protein mislocalization, such as Parkinson’s and Alzheimer’s.
In summary, recombinant TIMM21 is vital for dissecting mitochondrial import machinery, understanding disease pathogenesis, and developing strategies to target mitochondrial dysfunction. Its applications span basic research, diagnostics, and therapeutic innovation.
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