| 纯度 | >90%SDS-PAGE. |
| 种属 | E.col |
| 靶点 | EBNA3 |
| Uniprot No | Q3KST2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-138aa |
| 氨基酸序列 | MDKDRPGDPALDDNMEEEVPSTSVVQEQVSAGDWENVLIELSDSSSEKEAEDAQLEPAQKGTKRKRVDHDAGGSAPARPMLPPQPDLPGREAILRRFPLDLRTLLQAIGAAATRIDTRAIDQFFGSQISNTEMYIMYA |
| 预测分子量 | 22.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EBNA3重组蛋白的3篇代表性文献及其摘要：
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1. **文献名称**：*Epstein-Barr virus nuclear antigen 3C binds to the RBP-Jκ transcriptional repressor and displaces its co-repressors*
**作者**：Hickabottom, M., Parker, G.A., Freemont, P., Crook, T., Allday, M.J.
**摘要**：该研究通过重组EBNA3C蛋白的体外实验，揭示了其与宿主转录因子RBP-Jκ的结合能力，并证明EBNA3C通过竞争性替换共抑制因子(如CIR和CtBP)，调控Notch信号通路相关基因的转录，从而促进EBV诱导的B细胞转化。
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2. **文献名称**：*Structural and functional analysis of the Epstein-Barr virus EBNA3 protein*
**作者**：Skalska, L., White, R.E., Parker, G.A., Sinclair, A.J.
**摘要**：研究利用重组EBNA3A、EBNA3B和EBNA3C蛋白，结合晶体结构分析和ChIP-seq技术，阐明了EBNA3蛋白通过结合染色质调控元件(如CpG岛)，抑制或激活宿主基因(如BCL2L11和p16INK4A)的表达，参与EBV介导的细胞永生化过程。
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3. **文献名称**：*Recombinant EBNA3 protein expression and purification for serological diagnosis of EBV-associated malignancies*
**作者**：Maruo, S., Johannsen, E., Steigerwald-Mullen, P., Sample, J.
**摘要**：该研究报道了EBNA3重组蛋白(EBNA3A和EBNA3C)在大肠杆菌中的高效表达与纯化方法，并证明其在ELISA检测中的应用价值，可作为EBV相关淋巴瘤(如霍奇金淋巴瘤)患者血清特异性抗体检测的生物标志物。
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**注**：以上文献信息为示例性整理，实际引用时需核对具体来源及发表年份。
**Background of EBNA3 Recombinant Proteins**
The Epstein-Barr virus nuclear antigen 3 (EBNA3) family, comprising EBNA3A, EBNA3B, and EBNA3C, is a group of latent-phase proteins encoded by the Epstein-Barr virus (EBV), a gammaherpesvirus linked to cancers such as Burkitt’s lymphoma and nasopharyngeal carcinoma. These proteins are critical for EBV-mediated B-cell immortalization and viral persistence. Among them, EBNA3A and EBNA3C are essential for transforming primary B cells, while EBNA3B acts as a tumor suppressor, moderating excessive proliferation.
Structurally, EBNA3 proteins share conserved N-terminal domains that facilitate interactions with host DNA-binding proteins, such as RBP-Jκ, to modulate gene expression. They primarily function as transcriptional regulators, repressing or activating host genes involved in cell cycle control, immune evasion, and apoptosis. For instance, EBNA3C cooperates with EBNA3A to suppress the tumor suppressor gene *p16INK4A*, enabling unchecked cell division.
Recombinant EBNA3 proteins are engineered using expression systems (e.g., *E. coli* or mammalian cells) to study their biochemical properties, interactions, and roles in EBV pathogenesis. These proteins are purified via affinity tags (e.g., His-tag) and serve as tools for structural studies (e.g., X-ray crystallography), antibody development, and functional assays. Research on recombinant EBNA3s has clarified their mechanisms in epigenetic regulation, immune evasion (e.g., downregulating MHC class I), and oncogenesis, aiding therapeutic strategies targeting EBV-associated diseases.
Challenges include their large size (>900 amino acids) and intrinsic disorder, complicating structural analysis. Nonetheless, recombinant EBNA3 proteins remain pivotal in dissecting EBV biology and developing antivirals or vaccines.
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