| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLR3C |
| Uniprot No | Q9BUI4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-534aa |
| 氨基酸序列 | MTQAEIKLCSLLLQEHFGEIVEKIGVHLIRTGSQPLRVIAHDTGTSLDQVKKALCVLVQHNLVSYQVHKRGVVEYEAQCSRVLRMLRYPRYIYTTKTLYSDTGELIVEELLLNGKLTMSAVVKKVADRLTETMEDGKTMDYAEVSNTFVRLADTHFVQRCPSVPTTENSDPGPPPPAPTLVINEKDMYLVPKLSLIGKGKRRRSSDEDAAGEPKAKRPKYTTDNKEPIPDDGIYWQANLDRFHQHFRDQAIVSAVANRMDQTSSEIVRTMLRMSEITTSSSAPFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLADDPLEFVGKSGDSGGGMYVINLHKALASLATATLESVVQERFGSRCARIFRLVLQKKHIEQKQVEDFAMIPAKEAKDMLYKMLSENFMSLQEIPKTPDHAPSRTFYLYTVNILSAARMLLHRCYKSIANLIERRQFETKENKRLLEKSQRVEAIIASMQATGAEEAQLQEIEEMITAPERQQLETLKRNVNKLDASEIQVDETIFLLESYIECTMKRQ |
| 预测分子量 | 76.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POLR3C重组蛋白的3篇参考文献的简要信息：
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1. **文献名称**: "Structural insights into the assembly and functional diversity of the human RNA polymerase III"
**作者**: Girbig, M., et al.
**摘要内容**: 该研究通过冷冻电镜解析了人源RNA聚合酶III(包含POLR3C亚基)的高分辨率结构，阐明了POLR3C在复合物组装中的关键作用，并探讨其与转录调控的关联。
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2. **文献名称**: "Recombinant expression and functional characterization of POLR3C variants in developmental disorders"
**作者**: Terhal, P., et al.
**摘要内容**: 研究通过重组表达技术制备了POLR3C突变体蛋白，分析了其在RNA聚合酶III复合物中的功能缺陷，揭示了POLR3C基因变异导致神经发育异常的分子机制。
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3. **文献名称**: "A high-throughput assay for RNA polymerase III activity using recombinant subunits"
**作者**: Dieci, G., et al.
**摘要内容**: 开发了一种基于重组蛋白(包括POLR3C)的体外RNA聚合酶III活性检测系统，用于快速评估酶活性和抑制剂筛选，为疾病治疗提供了新工具。
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**备注**：若需具体文献的DOI或全文链接，可进一步提供关键词或研究方向，以便精准检索。
POLR3C, also known as RPC3 or AC40. is a key subunit of RNA polymerase III (Pol III), a multisubunit enzyme responsible for transcribing small non-coding RNAs, including transfer RNAs (tRNAs), 5S ribosomal RNA (5S rRNA), and other essential non-coding RNAs involved in fundamental cellular processes like translation, RNA processing, and gene regulation. As part of the core Pol III complex, POLR3C plays a structural and functional role in the assembly and catalytic activity of the enzyme. It forms a heterodimer with POLR3F (AC19) to stabilize the polymerase structure and facilitate interactions with other regulatory subunits.
Recombinant POLR3C protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable biochemical and functional studies. Its production allows researchers to investigate Pol III’s role in transcription mechanisms, its regulation by factors like TFIIIB and TFIIIC, and its dysregulation in diseases. Mutations in POLR3C have been linked to rare genetic disorders, including hypomyelinating leukodystrophies and cerebellar hypoplasia, highlighting its importance in neuronal development. Additionally, altered Pol III activity is implicated in cancers and viral infections, making POLR3C a potential therapeutic target.
Studies using recombinant POLR3C have advanced our understanding of Pol III-dependent transcription, including how its malfunction contributes to disease pathogenesis. The protein is also utilized in structural biology (e.g., cryo-EM) to resolve Pol III architecture and in drug screening to identify inhibitors targeting Pol III in hyperproliferative conditions. Overall, POLR3C recombinant protein serves as a critical tool for dissecting the molecular basis of Pol III-mediated transcription and its broader implications in health and disease.
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