| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | UGT78D1 |
| Uniprot No | Q9S9P6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-453aa |
| 氨基酸序列 | MTKFSEPIRDSHVAVLAFFPVGAHAGPLLAVTRRLAAASPSTIFSFFNTARSNASLFSSDHPENIKVHDVSDGVPEGTMLGNPLEMVELFLEAAPRIFRSEIAAAEIEVGKKVTCMLTDAFFWFAADIAAELNATWVAFWAGGANSLCAHLYTDLIRETIGLKDVSMEETLGFIPGMENYRVKDIPEEVVFEDLDSVFPKALYQMSLALPRASAVFISSFEELEPTLNYNLRSKLKRFLNIAPLTLLSSTSEKEMRDPHGCFAWMGKRSAASVAYISFGTVMEPPPEELVAIAQGLESSKVPFVWSLKEKNMVHLPKGFLDRTREQGIVVPWAPQVELLKHEAMGVNVTHCGWNSVLESVSAGVPMIGRPILADNRLNGRAVEVVWKVGVMMDNGVFTKEGFEKCLNDVFVHDDGKTMKANAKKLKEKLQEDFSMKGSSLENFKILLDEIVKV |
| 预测分子量 | 55.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UGT78D1重组蛋白的3篇代表性文献的简要信息，涵盖其功能研究和应用方向：
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1. **文献名称**：*Functional Characterization of Recombinant UGT78D1 in Arabidopsis thaliana as a Flavonoid Glucosyltransferase*
**作者**：Jones, R. et al.
**摘要**：本研究在大肠杆菌中成功表达并纯化了拟南芥UGT78D1重组蛋白。酶活分析表明，该蛋白催化黄酮类化合物(如槲皮素和山奈酚)的3-O-葡萄糖基化反应，揭示了其在植物花色苷合成中的关键作用。研究还通过突变实验鉴定了催化活性必需的关键氨基酸残基。
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2. **文献名称**：*Heterologous Expression and Substrate Screening of Soybean UGT78D1 Homolog in Saccharomyces cerevisiae*
**作者**：Zhang, L. et al.
**摘要**：通过酵母表达系统制备大豆UGT78D1同源重组蛋白，并测试其对多种酚类底物的催化活性。结果显示该蛋白优先催化异黄酮(如染料木素)的糖基化，表明其在豆科植物次生代谢中的特异性功能。研究为利用该酶合成糖苷类活性分子提供了依据。
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3. **文献名称**：*Structural Insights into UGT78D1 Catalytic Mechanism via Recombinant Protein Crystallography*
**作者**：Smith, K. et al.
**摘要**：利用昆虫细胞系统表达并结晶了UGT78D1重组蛋白，通过X射线衍射解析其三维结构。结合分子对接实验，揭示了底物结合口袋的构象及糖基供体(UDP-glucose)的结合模式，为理性设计高效糖基转移酶提供了结构基础。
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**备注**：上述文献信息为模拟示例，实际研究中建议通过数据库(如PubMed、Web of Science)检索具体文献。若需进一步分析某篇文献，可提供完整标题或DOI。
UGT78D1 is a plant-derived uridine diphosphate (UDP)-dependent glycosyltransferase belonging to the UGT superfamily, which catalyzes the transfer of sugar moieties from activated nucleotide sugars to diverse acceptor molecules. Predominantly studied in *Arabidopsis thaliana*, UGT78D1 plays a critical role in flavonoid metabolism, particularly in the glycosylation of anthocyanins and proanthocyanidins—key pigments and defense compounds influencing plant coloration, UV protection, and stress responses. This enzyme is implicated in enhancing the stability, solubility, and bioactivity of flavonoid aglycones, thereby modulating their physiological functions and storage in plant tissues.
Recombinant UGT78D1 protein is typically produced via heterologous expression systems, such as *E. coli* or yeast, enabling large-scale purification for biochemical and structural studies. Its recombinant form retains catalytic activity, allowing researchers to characterize substrate specificity, kinetic parameters, and regulatory mechanisms. Studies have revealed UGT78D1’s preference for UDP-glucose as a sugar donor and its ability to glycosylate flavonols like kaempferol and quercetin, contributing to the diversification of flavonoid derivatives in plants.
Research on recombinant UGT78D1 has advanced understanding of glycosylation’s role in plant secondary metabolism and stress adaptation. It also holds biotechnological potential, such as engineering crops with improved stress tolerance or modified pigmentation, or synthesizing bioactive glycosides for pharmaceutical applications. However, structural and functional nuances, including its interaction with other metabolic enzymes, remain areas of active exploration. Overall, UGT78D1 exemplifies the intersection of plant biochemistry, enzymology, and applied biotechnology.
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