| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | QPCTL |
| Uniprot No | Q9NXS2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 212-382aa |
| 氨基酸序列 | AAPVTLQLLFLDGEEALKEWGPKDSLYGSRHLAQLMESIPHSPGPTRIQAIELFMLLDLLGAPNPTFYSHFPRTVRWFHRLRSIEKRLHRLNLLQSHPQEVMYFQPGEPFGSVEDDHIPFLRRGVPVLHLISTPFPAVWHTPADTEVNLHPPTVHNLCRILAVFLAEYLGL |
| 预测分子量 | 35.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于QPCTL重组蛋白的3篇文献示例(内容基于研究领域常见主题，非真实文献)：
1. **文献名称**：Structural characterization of recombinant human QPCTL and its role in pyroglutamate formation
**作者**：Smith A, et al.
**摘要**：本研究通过大肠杆菌表达系统重组生产人源QPCTL蛋白，解析其晶体结构，揭示其催化焦谷氨酸形成的活性位点及底物结合机制。
2. **文献名称**：Enzymatic activity profiling of QPCTL in cancer cell migration and invasion
**作者**：Zhang L, et al.
**摘要**：利用昆虫细胞表达重组QPCTL蛋白，发现其通过修饰趋化因子CD26/DPP4的焦谷氨酸化促进肿瘤细胞迁移，提示其作为抗癌靶点的潜力。
3. **文献名称**：QPCTL-mediated post-translational modification in neurodegenerative disease models
**作者**：Johnson R, et al.
**摘要**：研究重组QPCTL在神经细胞中的表达，证实其通过β-淀粉样蛋白焦谷氨酸化加剧阿尔茨海默病模型中的神经毒性。
(注：以上为模拟文献，实际研究中请查询具体数据库获取真实文献。)
**Background of QPCTL Recombinant Protein**
QPCTL (glutaminyl peptide cyclotransferase-like) is a member of the QC enzyme family, which catalyzes the cyclization of N-terminal glutaminyl or glutamyl residues into pyroglutamate (pGlu) in peptides and proteins. This post-translational modification plays a critical role in stabilizing protein structures, modulating biological activity, and influencing resistance to proteolytic degradation. QPCTL shares structural and functional similarities with its homolog QPCT (glutaminyl cyclase), but it exhibits distinct substrate preferences and tissue expression patterns, suggesting specialized roles in physiological and pathological processes.
The enzyme has garnered attention for its involvement in neuroinflammatory and neurodegenerative diseases. For instance, QPCTL contributes to the formation of pGlu-modified amyloid-beta (Aβ) peptides, a hallmark of Alzheimer’s disease, by promoting Aβ aggregation and toxicity. It also regulates chemokine activity (e.g., CCL2. CX3CL1) by modifying their N-termini, thereby influencing immune cell recruitment and inflammation.
Recombinant QPCTL protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and enzymatic activity. This engineered protein serves as a vital tool for studying pGlu-dependent mechanisms, screening inhibitors for therapeutic development, and elucidating structural features of the enzyme. Its applications extend to drug discovery, particularly in targeting neurodegenerative disorders and chronic inflammatory conditions.
Research on QPCTL continues to uncover its regulatory roles in immunity, metabolism, and disease progression, positioning it as a promising biomarker and therapeutic target.
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