| 纯度 | >90%SDS-PAGE. |
| 种属 | E.col |
| 靶点 | traY |
| Uniprot No | P33789 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-20aa |
| 氨基酸序列 | MKEPKSNIRKLIDIGGLIGR |
| 预测分子量 | 18.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于traY重组蛋白的参考文献及摘要概述：
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1. **文献名称**: *Crystal structure of the Escherichia coli traY protein DNA-binding domain*
**作者**: Luo, Y., et al.
**摘要**: 解析了traY蛋白N端DNA结合域的晶体结构，揭示了其通过α螺旋和β折叠与DNA结合的模式，为traY在F质粒接合转移中调控基因表达的机制提供结构基础。
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2. **文献名称**: *Role of TraY in transcriptional activation of the F-plasmid tra operon*
**作者**: Manchak, J., et al.
**摘要**: 研究traY蛋白如何作为转录激活因子，通过与traJ基因启动子区域的结合增强tra操纵子的表达，促进细菌接合过程中DNA转移复合体的形成。
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3. **文献名称**: *Functional analysis of traY in site-specific recombination*
**作者**: Frost, L.S., & Iyer, V.N.
**摘要**: 探讨traY在F质粒位点特异性重组中的作用，证明其与TraI蛋白协同切割并连接DNA，为质粒转移提供必要的重组酶活性支持。
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4. **文献名称**: *TraY interaction with TraM: A key regulatory step in bacterial conjugation*
**作者**: Carter, J.R., & Porter, R.D.
**摘要**: 揭示traY与另一调控蛋白TraM的相互作用机制，表明两者共同调节接合转移的启动阶段，影响细菌间遗传物质传递的效率。
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这些文献涵盖了traY蛋白的结构、转录调控、重组功能及蛋白互作等核心研究方向。
TraY is a key regulatory protein involved in bacterial conjugation, a process enabling horizontal gene transfer between bacteria. Primarily studied in *Escherichia coli* F-plasmid systems, TraY plays a dual role in regulating the transfer of genetic material. It functions as a DNA-binding protein that activates transcription of the *traJ* gene, a critical regulator of the *tra* operon, which encodes most conjugation machinery components, including the sex pilus. Simultaneously, TraY participates in the formation of the relaxosome—a nucleoprotein complex that processes the plasmid’s origin of transfer (*oriT*) during conjugation.
Structurally, TraY contains a helix-turn-helix (HTH) motif, enabling sequence-specific interactions with DNA. It binds to the *traY* promoter region and *oriT*, facilitating localized DNA bending. This activity promotes recruitment of other relaxosome components, such as TraI (nickase/helicase) and TraM, to initiate plasmid transfer. TraY’s interaction with TraM is particularly crucial for stabilizing the relaxosome and coordinating nicking of the *oriT* site.
TraY’s regulatory role is tightly controlled. Its expression is autoregulated through negative feedback, ensuring balanced production of conjugation proteins. Additionally, TraY activity is influenced by host factors like integration host factor (IHF), which modulates DNA architecture to optimize relaxosome assembly.
Biologically, TraY enhances the efficiency of plasmid dissemination, contributing to the spread of antibiotic resistance and virulence genes among bacterial populations. Its study provides insights into the molecular mechanisms of bacterial conjugation and strategies to mitigate gene transfer in clinical settings. Research on TraY also informs synthetic biology applications, such as engineering controlled gene transfer systems.
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