| 纯度 | >90%SDS-PAGE. |
| 种属 | Rat |
| 靶点 | TRY 4 |
| Uniprot No | P12788 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-247aa |
| 氨基酸序列 | IVGGYTCPKHLVPYQVSLHDGISHQCGGSLISDQWVLSAAHCYKRKLQVRLGEHNIHVLEGGEQFIDAEKIIRHPEYNKDTLDNDIMLIKLKSPAVLNSQVSTVSLPRSCASTDAQCLVSGWGNTVSIGGKYPALLQCLEAPVLSASSCKKSYPGQITSNMFCLGFLEGGKDSCDGDSGGPVVCNGEIQGIVSWGSVCAMRGKPGVYTKVCNYLSWIQETMANN |
| 预测分子量 | 26.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下提供的参考文献为示例性质，具体研究内容需根据实际文献调整。由于“TRY4重组蛋白”的命名在公开数据库中检索匹配度较低，可能涉及特定研究领域的非标准命名，建议结合具体研究背景进一步验证。
1. **文献名称**： *Recombinant TRY4 Protein Enhances Wound Healing in Diabetic Mouse Models*
**作者**：Chen L., et al.
**摘要**：本研究构建了TRY4重组蛋白，通过体外细胞实验证实其可促进成纤维细胞迁移。在小鼠糖尿病创面模型中，局部应用TRY4显著加速上皮再生，可能通过激活EGFR信号通路发挥作用。
2. **文献名称**：*Structural Characterization of TRY4 Recombinant Protein and Its Role in Immune Regulation*
**作者**：Kim S., et al.
**摘要**：利用X射线晶体学解析TRY4重组蛋白的三维结构，发现其具有独特的β-折叠结构域。体外实验表明，TRY4可抑制巨噬细胞过度炎症反应，提示其在自身免疫性疾病中的潜在治疗价值。
3. **文献名称**：*TRY4 Recombinant Protein as a Novel Biomarker for Early-Stage Hepatocellular Carcinoma*
**作者**：Wang Y., et al.
**摘要**：通过ELISA检测血清TRY4水平，发现其在肝癌患者中显著升高(AUC=0.89)。重组蛋白的制备采用哺乳动物表达系统，质谱验证其糖基化修饰与天然蛋白高度一致。
4. **文献名称**：*High-Yield Production of TRY4 Recombinant Protein in E. coli and Its Antimicrobial Activity Analysis*
**作者**：Rodriguez P., et al.
**摘要**：优化大肠杆菌表达系统实现TRY4可溶性表达(纯度>95%)。体外抑菌实验显示其对革兰氏阴性菌(如大肠杆菌)具有选择性抑制作用，最小抑菌浓度(MIC)为32μg/mL。
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**注意**：以上文献信息为模拟数据，实际研究中请通过PubMed、Web of Science等平台以“recombinant protein TRY4”“TRY4 expression”等关键词检索，或结合具体物种(如植物TRY蛋白酶)缩小范围。若涉及基因命名歧义，需确认基因官方符号(如拟南芥AtTRY4与哺乳动物蛋白区别)。
**Background of TRPV4 Recombinant Protein**
TRPV4 (Transient Receptor Potential Vanilloid 4) is a calcium-permeable ion channel belonging to the TRP superfamily, which plays critical roles in sensory transduction and cellular responses to mechanical, thermal, and chemical stimuli. Discovered in the early 2000s, TRPV4 is widely expressed in tissues such as the kidneys, lungs, skin, and nervous system, where it regulates osmotic balance, nociception, and mechanosensation. Its activation is triggered by diverse stimuli, including hypotonicity, moderate heat (27–34°C), and endogenous ligands like arachidonic acid metabolites.
Structurally, TRPV4 comprises six transmembrane domains with intracellular N- and C-termini. The C-terminal region contains binding sites for modulators like calmodulin and PIP2. influencing channel gating and trafficking. Dysregulation of TRPV4 is linked to pathologies such as neuropathic pain, skeletal dysplasia, and pulmonary edema, making it a therapeutic target.
Recombinant TRPV4 proteins are engineered to study its function and interactions. Produced via heterologous expression systems (e.g., HEK293 or insect cells), these proteins retain native channel properties, enabling *in vitro* studies on ion permeability, drug screening, and structure-function analysis. Tagged versions (e.g., His-tag or GFP-fusion) facilitate purification and visualization. Research utilizing TRPV4 recombinant proteins has advanced understanding of its role in disease mechanisms and spurred development of selective agonists/antagonists, highlighting its potential in treating channelopathies and inflammatory conditions.
In summary, TRPV4 recombinant proteins serve as essential tools for dissecting the channel’s physiological and pathological roles, bridging molecular insights to therapeutic innovation.
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