Human transforming growth factor α,TGF-α ELISA Kit

中文名： 人转化生长因子α(TGF-α)ELISA Kit

货号: IPD-E04724h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人转化生长因子α(TGF-α)酶联免疫试剂盒（CSB-E04724h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TGFA含量。TGFA（转化生长因子α）是一种促有丝分裂肽，与EGFR结合并激活RAS/RAF/MAPK通路，促进细胞增殖、侵袭和转移。其研究机制涉及与SDCBP和SNTA1 PDZ结构域的相互作用，以及通过microRNA和ADAM17/TACE等调控因子影响肿瘤生长和转移。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，灵敏度为15.6 pg/mL。该产品广泛应用于细胞功能研究、肿瘤机制探索、药物作用评估等科研领域，为体外定量分析TGF-α提供可靠工具。TGF-α在肿瘤发生、伤口愈合及胚胎发育等研究中具有重要价值。适用于检测不同生理或实验条件下样本的TGF-α表达水平。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
TGFA; Protransforming growth factor alpha
缩写：
TGFA
Uniprot No.：
P01135
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)504.002492.653490.323514.270489.119506.002SD0.0360.0410.0330.0560.0520.048CV(%)4.2764.8983.9666.7636.2285.701

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

85-99

1:2

Average %

Range %

87-105

1:4

Average %

Range %

92-99

1:8

Average %

Range %

86-98

回收率：

The recovery of human TGF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	93	86-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
4000	1.875	1.807	1.841	1.711
2000	1.523	1.495	1.509	1.379
1000	1.170	1.126	1.148	1.018
500	0.854	0.840	0.847	0.717
250	0.626	0.614	0.620	0.490
125	0.413	0.422	0.418	0.288
62.5	0.289	0.296	0.293	0.163
0	0.131	0.128	0.130