| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LMP1 |
| Uniprot No | Q13114 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-568aa |
| 氨基酸序列 | MESSKKMDSPGALQTNPPLKLHTDRSAGTPVFVPEQGGYKEKFVKTVEDKYKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKDKVFKDNCCKREILALQIYCRNESRGCAEQLMLGHLLVHLKNDCHFEELPCVRPDCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIALQKHEDTDCPCVVVSCPHKCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIKDDTIFIKVIVDTSDLPDP |
| 预测分子量 | 64.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于LMP1重组蛋白的参考文献示例(内容基于典型研究方向模拟，非真实文献)：
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1. **文献名称**：*High-Yield Expression and Purification of Epstein-Barr Virus LMP1 Transmembrane Domain in Insect Cells*
**作者**：Miller, A. et al.
**摘要**：报道利用杆状病毒-昆虫细胞系统高效表达LMP1跨膜结构域，并通过亲和层析纯化获得可溶性蛋白，用于研究其自激活信号传导机制。
2. **文献名称**：*Recombinant LMP1 Cytoplasmic Tail Triggers NF-κB Signaling in vitro*
**作者**：Chen, L. & Wang, Y.
**摘要**：通过大肠杆菌表达LMP1胞内段重组蛋白，证明其直接结合TRAF分子并激活NF-κB通路，揭示其在EBV相关肿瘤发生中的关键作用。
3. **文献名称**：*Structural Insights into LMP1 Oligomerization via Recombinant Protein Crystallography*
**作者**：Tanaka, K. et al.
**摘要**：利用重组表达的LMP1胞内结构域进行晶体结构解析，揭示其通过C末端结构域寡聚化激活下游信号通路的分子机制。
4. **文献名称**：*Development of a LMP1-Based Recombinant Vaccine Candidate Using Mammalian Expression Systems*
**作者**：Gupta, R. et al.
**摘要**：优化哺乳动物细胞表达系统生产全长LMP1重组蛋白，评估其作为EBV疫苗成分的免疫原性及抗体中和能力。
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**注**：以上文献为示例性质，实际研究中建议通过PubMed、Web of Science等数据库以关键词“LMP1 recombinant protein”或“LMP1 expression”检索最新文章。
LMP1 (Latent Membrane Protein 1) is a key oncogenic protein encoded by the Epstein-Barr virus (EBV), a herpesvirus linked to several malignancies, including nasopharyngeal carcinoma, Hodgkin’s lymphoma, and gastric cancer. As a transmembrane protein, LMP1 mimics a constitutively active tumor necrosis factor receptor (TNFR), playing a central role in EBV-mediated cellular transformation and immune evasion. Its structure includes a short N-terminal cytoplasmic tail, six transmembrane domains, and a long C-terminal cytoplasmic region containing signaling motifs that recruit tumor necrosis factor receptor-associated factors (TRAFs) and other adaptors. These interactions activate downstream pathways such as NF-κB, MAPK, and PI3K/Akt, driving cell proliferation, survival, and inflammation.
Recombinant LMP1 proteins are artificially engineered versions produced in heterologous expression systems (e.g., bacteria, yeast, or mammalian cells) for functional studies. These proteins retain critical domains required for signaling but are often modified with tags (e.g., His, FLAG) to facilitate purification and detection. Researchers use recombinant LMP1 to dissect its molecular mechanisms, screen inhibitors, or develop diagnostic tools. For example, studies with purified LMP1 have revealed its role in promoting epithelial-mesenchymal transition (EMT) or modulating immune checkpoints like PD-L1. Challenges in producing functional recombinant LMP1 arise from its hydrophobic transmembrane regions, which complicate solubility and proper folding in vitro. Despite this, advancements in cell-free systems or membrane-mimicking environments have improved protein stability. Current research focuses on leveraging recombinant LMP1 to design targeted therapies or vaccines against EBV-associated cancers, highlighting its dual role as a pathogenic driver and a therapeutic vulnerability.
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