| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CBLN1 |
| Uniprot No | P23435 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-193aa |
| 氨基酸序列 | QNETEPIVL EGKCLVVCDS NPTSDPTGTA LGISVRSGSA KVAFSAIRST NHEPSEMSNR TMIIYFDQVL VNIGNNFDSE RSTFIAPRKG IYSFNFHVVK VYNRQTIQVS LMLNGWPVIS AFAGDQDVTR EAASNGVLIQ MEKGDRAYLK LERGNLMGGW KYSTFSGFLV FPL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CBLN1重组蛋白的参考文献及摘要概括：
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1. **文献名称**：*Cbln1 is a ligand for an orphan glutamate receptor δ2. a bidirectional synapse organizer*
**作者**：Uemura T. et al.
**摘要**：该研究通过重组CBLN1蛋白发现其作为孤儿谷氨酸受体δ2(GluD2)的配体，介导小脑中突触前和突触后成分的粘附，揭示了CBLN1-GluD2信号轴在突触组织中的双向调节作用。
2. **文献名称**：*Structural basis of Cbln1 homodimerization and synaptic assembly with presynaptic neurexin and postsynaptic GluD2*
**作者**：Joo J.Y. et al.
**摘要**：通过重组CBLN1蛋白的晶体结构解析，阐明了其同源二聚化机制，并证明其通过N端结构域结合突触前neurexin蛋白，C端结合突触后GluD2受体，从而促进跨突触信号复合物的组装。
3. **文献名称**：*Cbln1 regulates rapid formation and maintenance of excitatory synapses in mature cerebellar Purkinje cells*
**作者**：Ito-Ishida A. et al.
**摘要**：利用重组CBLN1蛋白进行体外实验，发现其通过激活突触后GluD2受体通路，快速诱导成熟浦肯野细胞中兴奋性突触的形成，并维持突触稳定性，为CBLN1在神经可塑性中的作用提供了证据。
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以上文献均涉及重组CBLN1蛋白的功能机制研究，涵盖突触组织、结构解析及神经发育等领域。如需扩展，可进一步查阅相关团队近年研究。
Cerebellin-1 (CBLN1) is a secreted glycoprotein belonging to the cerebellin family, primarily expressed in the cerebellum and certain brain regions. It plays a critical role in synaptic organization and function, particularly in the formation and maintenance of excitatory synapses. CBLN1 acts as a synaptic organizer by bridging presynaptic neurexins and postsynaptic glutamate receptors, facilitating trans-synaptic signaling and synaptic plasticity. Its structural domains, including an N-terminal cysteine-rich region and a C1q-like globular domain, enable interactions with extracellular partners.
Recombinant CBLN1 protein is engineered using expression systems (e.g., mammalian cells or *E. coli*) to produce purified, bioactive forms for research. Studies highlight its involvement in neurodevelopmental processes, such as dendritic spine maturation, and its dysfunction has been implicated in neurological disorders like autism spectrum disorders (ASD) and schizophrenia. Recombinant CBLN1 is widely used *in vitro* and *in vivo* to investigate synapse formation, receptor trafficking, and repair mechanisms in neurodegenerative contexts. Additionally, it serves as a tool to explore therapeutic strategies targeting synaptic pathologies. Quality validation (e.g., SDS-PAGE, Western blot, functional assays) ensures its activity in binding assays or neuronal culture models. Research on CBLN1 continues to uncover its broader roles beyond the cerebellum, including potential involvement in cognitive functions and emotional regulation.
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