| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAP2 |
| Uniprot No | P40123 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-477aa |
| 氨基酸序列 | ANMQGLVER LERAVSRLES LSAESHRPPG NCGEVNGVIA GVAPSVEAFD KLMDSMVAEF LKNSRILAGD VETHAEMVHS AFQAQRAFLL MASQYQQPHE NDVAALLKPI SEKIQEIQTF RERNRGSNMF NHLSAVSESI PALGWIAVSP KPGPYVKEMN DAATFYTNRV LKDYKHSDLR HVDWVKSYLN IWSELQAYIK EHHTTGLTWS KTGPVASTVS AFSVLSSGPG LPPPPPPLPP PGPPPLFENE GKKEESSPSR SALFAQLNQG EAITKGLRHV TDDQKTYKNP SLRAQGGQTQ SPTKSHTPSP TSPKSYPSQK HAPVLELEGK KWRVEYQEDR NDLVISETEL KQVAYIFKCE KSTIQIKGKV NSIIIDNCKK LGLVFDNVVG IVEVINSQDI QIQVMGRVPT ISINKTEGCH IYLSEDALDC EIVSAKSSEM NILIPQDGDY REFPIPEQFK TAWDGSKLIT EPAEIMA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAP2重组蛋白的3篇参考文献示例，涵盖其功能研究与制备应用：
1. **《Cyclase-associated protein 2 regulates actin dynamics and mTORC2 signaling in glioblastoma cells》**
- 作者：Smith, J. et al.
- 摘要：研究通过重组CAP2蛋白揭示其在胶质母细胞瘤细胞中调控肌动蛋白动态和mTORC2信号通路的机制，为肿瘤侵袭性提供新见解。
2. **《Recombinant CAP2 purification and its role in cell migration》**
- 作者：Li, X. & Wang, H.
- 摘要：报道大肠杆菌表达系统制备CAP2重组蛋白的优化方法，并证实其在体外细胞迁移实验中对肌动蛋白重塑的关键作用。
3. **《Structural insights into CAP2-mediated actin depolymerization》**
- 作者：Garcia, R. et al.
- 摘要：利用重组CAP2蛋白进行冷冻电镜分析，解析其与肌动蛋白丝结合的分子结构，阐明其促进肌动蛋白解聚的功能域特征。
注：以上文献为示例，实际引用时建议通过学术数据库(如PubMed、Web of Science)检索最新研究。若需具体文献，可补充关键词或研究场景进一步筛选。
**Background of CAP2 Recombinant Protein**
CAP2 (Cyclase-Associated Protein 2) is a conserved eukaryotic protein involved in regulating actin cytoskeleton dynamics, a critical process for cell motility, morphology, and intracellular trafficking. It interacts with actin monomers and cooperates with cofilin to promote actin filament disassembly, facilitating cytoskeletal remodeling. CAP2 also associates with signaling molecules like Rho GTPases and adenylate cyclase, linking cytoskeletal regulation to cellular signaling pathways.
Biologically, CAP2 plays roles in development and tissue homeostasis. Studies in model organisms, such as *Drosophila* and mice, highlight its importance in cardiac function, neuronal development, and muscle organization. CAP2 knockout mice exhibit dilated cardiomyopathy, skeletal myopathy, and neurological abnormalities, underscoring its physiological relevance. Dysregulation of CAP2 has been implicated in human diseases, including cancer metastasis (via enhanced cell migration) and cardiovascular disorders.
Recombinant CAP2 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Purified CAP2 is utilized to investigate its biochemical interactions, structural features, and mechanisms in actin dynamics. Researchers employ it in assays like actin polymerization/depolymerization, protein-binding studies, and cellular overexpression/knockdown experiments to dissect its roles in health and disease.
Overall, CAP2 recombinant protein serves as a vital tool for understanding cytoskeletal regulation and developing therapeutic strategies targeting actin-related pathologies.
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