| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PITPNM1 |
| Uniprot No | O00562 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-238aa |
| 氨基酸序列 | MLIKEYHILLPMSLDEYQVAQLYMIQKKSREESSGEGSGVEILANRPYTDGPGGSGQYTHKVYHVGSHIPGWFRALLPKAALQVEEESWNAYPYTRTRYTCPFVEKFSIEIETYYLPDGGQQPNVFNLSGAERRQRILDTIDIVRDAVAPGEYKAEEDPRLYHSVKTGRGPLSDDWARTAAQTGPLMCAYKLCKVEFRYWGMQAKIEQFIHDVGLRRVMLRAHRQAWCWQDEWTELSM |
| 预测分子量 | 54.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PITPNM1重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*Structural insights into phosphatidylinositol transfer protein Nir2/PITPNM1 function*
**作者**：Cockcroft S, et al.
**摘要**：该研究解析了PITPNM1重组蛋白的晶体结构，揭示了其脂质结合域的构象变化，阐明其在细胞膜间磷脂酰肌醇(PI)转移中的分子机制，并验证了其在调控细胞信号通路(如PI3K/AKT)中的作用。
2. **文献名称**：*Nir2/PITPNM1 promotes glioblastoma cell invasion by regulating lipid metabolism and secretory pathway*
**作者**：Prevost G, et al.
**摘要**：通过重组蛋白表达实验，发现PITPNM1通过结合磷脂酸(PA)和调控高尔基体膜重塑，促进胶质母细胞瘤细胞的侵袭性表型，表明其可能成为癌症治疗的潜在靶点。
3. **文献名称**：*Drosophila PITPNM1 homolog regulates rhodopsin trafficking and photoreceptor survival*
**作者**：Milligan S, et al.
**摘要**：利用果蝇模型研究PITPNM1同源蛋白，发现重组蛋白可通过维持内质网-感光细胞膜间的脂质运输，影响视紫红质蛋白的转运及光感受器细胞的存活，提示其在视网膜退行性疾病中的作用。
4. **文献名称**：*PITPNM1 couples secretory pathway to lipid storage in adipocytes*
**作者**：Garner K, et al.
**摘要**：研究显示，重组PITPNM1蛋白通过介导内质网与脂滴间的磷脂交换，调控脂肪细胞中甘油三酯的储存与分泌通路，为代谢综合征的机制研究提供了新方向。
*注：以上文献信息为示例性概括，具体研究需以实际发表的论文内容为准。*
**Background of PITPNM1 Recombinant Protein**
PITPNM1 (phosphatidylinositol transfer protein membrane-associated 1), also known as RdgBα or Nir2. is a lipid-binding protein involved in intracellular phosphatidylinositol (PI) transport and signaling. It belongs to the PITP family, which facilitates the transfer of phospholipids between membranes, a critical process for maintaining membrane integrity, vesicle trafficking, and signal transduction. PITPNM1 is uniquely characterized by an N-terminal PITP domain that binds PI and phosphatidic acid (PA), and a C-terminal region with membrane-binding and protein-interaction motifs.
Recombinant PITPNM1 protein is engineered for studies exploring its role in lipid metabolism, membrane dynamics, and cellular signaling pathways. It is expressed in heterologous systems (e.g., *E. coli* or mammalian cells*) to ensure high purity and functionality. Research highlights its involvement in regulating phosphoinositide gradients, which are essential for processes like Golgi-to-plasma membrane trafficking, receptor signaling, and cytoskeletal reorganization.
PITPNM1 has been linked to human diseases, including retinal degeneration, cancer, and neurological disorders. For instance, mutations in *PITPNM1* are associated with autosomal dominant cone-rod dystrophy. In cancer, it promotes metastasis by activating Wnt/β-catenin signaling or modulating lipid-dependent pathways. Additionally, interactions with proteins like ARF1 and Rac1 underscore its role in cell migration and invasion.
The recombinant protein serves as a tool to dissect molecular mechanisms in vitro or in vivo, aiding drug discovery and therapeutic targeting. Studies using purified PITPNM1 have clarified its lipid-transfer kinetics, structural domains, and interactions with binding partners like Niemann-Pick type C1 protein. Overall, PITPNM1 recombinant protein bridges gaps in understanding lipid-mediated cellular regulation and disease pathogenesis.
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