| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APC |
| Uniprot No | P25054 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 960-1337aa |
| 氨基酸序列 | SNDSLNSVSSSDGYGKRGQMKPSIESYSEDDESKFCSYGQYPADLAHKIHSANHMDDNDGELDTPINYSLKYSDEQLNSGRQSPSQNERWARPKHIIEDEIKQSEQRQSRNQSTTYPVYTESTDDKHLKFQPHFGQQECVSPYRSRGANGSETNRVGSNHGINQNVSQSLCQEDDYEDDKPTNYSERYSEEEQHEEEERPTNYSIKYNEEKRHVDQPIDYSLKYATDIPSSQKQSFSFSKSSSGQSSKTEHMSSSSENTSTPSSNAKRQNQLHPSSAQSRSGQPQKAATCKVSSINQETIQTYCVEDTPICFSRCSSLSSLSSAEDEIGCNQTTQEADSANTLQIAEIKEKIGTRSAEDPVSEVPAVSQHPRTKSSRL |
| 预测分子量 | 49.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于APC重组蛋白的3篇代表性文献(虚构示例，仅供参考)：
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1. **文献名称**: "Recombinant APC Protein Inhibits Wnt/β-Catenin Signaling in Colorectal Cancer Cells"
**作者**: Smith JL, et al.
**摘要**: 研究报道了通过大肠杆菌重组表达的APC蛋白片段(包含β-catenin结合域)，可有效抑制结直肠癌细胞中Wnt信号通路活性，减少肿瘤细胞增殖，为靶向治疗提供实验依据。
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2. **文献名称**: "Structural Analysis of the APC Tumor Suppressor via Cryo-EM"
**作者**: Chen R, et al.
**摘要**: 利用冷冻电镜解析了全长APC重组蛋白的三维结构，揭示了其与β-catenin和微管结合的关键结构域，阐明了APC在细胞骨架调控和肿瘤抑制中的分子机制。
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3. **文献名称**: "Optimizing Expression and Purification of Functional APC Protein in Mammalian Systems"
**作者**: Gupta S, et al.
**摘要**: 开发了一种基于哺乳动物细胞的重组APC蛋白表达体系，优化后的蛋白具有天然磷酸化修饰，在体外实验中成功恢复APC缺失细胞的β-catenin降解功能。
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(注：以上文献为示例性内容，实际研究需查阅真实数据库如PubMed。)
**Background of Recombinant APC Protein**
The adenomatous polyposis coli (APC) protein, encoded by the *APC* gene, is a multifunctional tumor suppressor critical for regulating cell proliferation, migration, and apoptosis. It is best known for its role in the Wnt/β-catenin signaling pathway, where it forms a destruction complex to target β-catenin for proteasomal degradation, preventing uncontrolled cell growth. Mutations in the *APC* gene are strongly linked to familial adenomatous polyposis (FAP) and sporadic colorectal cancers, where truncated APC proteins lose their ability to regulate β-catenin, leading to oncogenic signaling.
Recombinant APC protein is produced using biotechnological methods, such as expression in *E. coli* or mammalian cell systems, to generate functional domains of APC (e.g., β-catenin-binding regions, microtubule-binding motifs) for research and therapeutic applications. These engineered proteins retain key biological activities, enabling studies on Wnt pathway mechanisms, cancer biology, and potential drug discovery. For instance, recombinant APC fragments have been explored to restore β-catenin regulation in APC-deficient cancer cells or to develop targeted therapies.
Beyond oncology, APC’s involvement in cytoskeletal organization and neuronal development has spurred interest in neurodegenerative and inflammatory diseases. Recombinant APC variants are also investigated as biomarkers for cancer diagnosis or as tools to study protein interactions. Challenges remain in stabilizing large APC domains due to their structural complexity, but advances in protein engineering continue to enhance their utility. Overall, recombinant APC proteins serve as vital tools for dissecting APC’s roles in health and disease, offering translational potential in precision medicine and molecular therapeutics.
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