| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADCY9 |
| Uniprot No | O60503 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1353aa |
| 氨基酸序列 | MASPPHQQLLHHHSTEVSCDSSGDSNSVRVKINPKQLSSNSHPKHCKYSISSSCSSSGDSGGVPRRVGGGGRLRRQKKLPQLFERASSRWWDPKFDSVNLEEACLERCFPQTQRRFRYALFYIGFACLLWSIYFAVHMRSRLIVMVAPALCFLLVCVGFFLFTFTKLYARHYAWTSLALTLLVFALTLAAQFQVLTPVSGRGDSSNLTATARPTDTCLSQVGSFSMCIEVLFLLYTVMHLPLYLSLCLGVAYSVLFETFGYHFRDEACFPSPGAGALHWELLSRGLLHGCIHAIGVHLFVMSQVRSRSTFLKVGQSIMHGKDLEVEKALKERMIHSVMPRIIADDLMKQGDEESENSVKRHATSSPKNRKKKSSIQKAPIAFRPFKMQQIEEVSILFADIVGFTKMSANKSAHALVGLLNDLFGRFDRLCEETKCEKISTLGDCYYCVAGCPEPRADHAYCCIEMGLGMIKAIEQFCQEKKEMVNMRVGVHTGTVLCGILGMRRFKFDVWSNDVNLANLMEQLGVAGKVHISEATAKYLDDRYEMEDGKVIERLGQSVVADQLKGLKTYLISGQRAKESRCSCAEALLSGFEVIDGSQVSSGPRGQGTASSGNVSDLAQTVKTFDNLKTCPSCGITFAPKSEAGAEGGAPQNGCQDEHKNSTKASGGPNPKTQNGLLSPPQEEKLTNSQTSLCEILQEKGRWAGVSLDQSALLPLRFKNIREKTDAHFVDVIKEDSLMKDYFFKPPINQFSLNFLDQELERSYRTSYQEEVIKNSPVKTFASPTFSSLLDVFLSTTVFLTLSTTCFLKYEAATVPPPPAALAVFSAALLLEVLSLAVSIRMVFFLEDVMACTKRLLEWIAGWLPRHCIGAILVSLPALAVYSHVTSEYETNIHFPVFTGSAALIAVVHYCNFCQLSSWMRSSLATVVGAGPLLLLYVSLCPDSSVLTSPLDAVQNFSSERNPCNSSVPRDLRRPASLIGQEVVLVFFLLLLLVWFLNREFEVSYRLHYHGDVEADLHRTKIQSMRDQADWLLRNIIPYHVAEQLKVSQTYSKNHDSGGVIFASIVNFSEFYEENYEGGKECYRVLNELIGDFDELLSKPDYSSIEKIKTIGATYMAASGLNTAQAQDGSHPQEHLQILFEFAKEMMRVVDDFNNNMLWFNFKLRVGFNHGPLTAGVIGTTKLLYDIWGDTVNIASRMDTTGVECRIQVSEESYRVLSKMGYDFDYRGTVNVKGKGQMKTYLYPKCTDHRVIPQHQLSISPDIRVQVDGSIGRSPTDEIANLVPSVQYVDKTSLGSDSSTQAKDAHLSPKRPWKEPVKAEERGRFGKAIEKDDCDETGIEEANELTKLNVSKSV |
| 预测分子量 | 150 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADCY9重组蛋白的3篇代表性文献的简要概括(注：内容为模拟示例，非真实文献)：
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1. **文献名称**: *Expression and Functional Characterization of Recombinant ADCY9 in Mammalian Cells*
**作者**: Smith J, et al.
**摘要**: 本研究在大肠杆菌和HEK293细胞中表达了重组ADCY9蛋白，优化了纯化条件并验证其酶活性。结果显示，重组ADCY9可被G蛋白信号调控，并参与细胞内cAMP水平的动态调节，为研究其生理功能提供了工具。
2. **文献名称**: *Structural Insights into ADCY9 Activation by Gβγ Subunits*
**作者**: Zhang Y, et al.
**摘要**: 通过冷冻电镜解析了重组ADCY9与Gβγ蛋白复合物的三维结构，揭示了Gβγ亚基特异性激活ADCY9的分子机制，并发现其与心血管疾病相关突变位点的潜在关联。
3. **文献名称**: *ADCY9 Polymorphisms Alter cAMP Signaling in Macrophages via Recombinant Protein Analysis*
**作者**: Lee H, et al.
**摘要**: 利用重组ADCY9蛋白结合基因编辑技术，发现特定单核苷酸多态性(SNPs)显著降低其催化活性，导致巨噬细胞炎症反应异常，提示ADCY9在免疫调节中的关键作用。
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以上内容基于领域内常见研究方向模拟，实际文献需通过数据库(如PubMed、Web of Science)检索确认。
**Background of ADCY9 Recombinant Protein**
Adenylyl cyclase 9 (ADCY9) is a membrane-bound enzyme belonging to the adenylate cyclase family, which catalyzes the conversion of ATP to cyclic adenosine monophosphate (cAMP), a critical secondary messenger in cellular signaling. ADCY9 is encoded by the *ADCY9* gene and is distinguished by its tissue-specific expression, primarily in the heart, lungs, and brain. It plays a role in regulating physiological processes such as cardiac function, immune responses, and metabolic pathways through cAMP-dependent signaling.
Recombinant ADCY9 protein is engineered using molecular cloning techniques, often expressed in mammalian or insect cell systems to ensure proper post-translational modifications and functional activity. This purified protein retains the catalytic domain and structural features of native ADCY9. enabling researchers to study its biochemical properties, interaction partners, and regulatory mechanisms in vitro.
Interest in ADCY9 has grown due to its association with diseases. For instance, genetic variants of *ADCY9* are linked to cardiovascular disorders, asthma, and diabetes. Additionally, ADCY9 has emerged as a potential therapeutic target; studies suggest its involvement in HDL-cholesterol metabolism, impacting atherosclerosis. The recombinant protein serves as a tool for high-throughput drug screening, structure-function analysis, and mechanistic studies to identify modulators (activators or inhibitors) of ADCY9 activity.
Recent research also highlights ADCY9's role in cancer progression and immune evasion, broadening its biomedical relevance. By utilizing recombinant ADCY9. scientists aim to unravel its signaling networks and develop targeted therapies. Quality control assays, such as enzymatic activity tests and Western blotting, ensure the recombinant protein's reliability for experimental and preclinical applications. Overall, ADCY9 recombinant protein is a vital resource for advancing understanding of cAMP-mediated pathways and their therapeutic exploitation.
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