Recombinant Human RYK protein

中文名： 相关受体酪氨酸激酶(RYK)重组蛋白
别名： RYK；JTK5A；Tyrosine-protein kinase RYK

货号: PA1000-9093

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	RYK
Uniprot No	P34925-1
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-227aa
氨基酸序列	MRGAARLGRP GRSCLPGARG LRAPPPPPLL LLLALLPLLP APGAAAAPAP RPPELQSASA GPSVSLYLSE DEVRRLIGLD AELYYVRNDL ISHYALSFSL LVPSETNFLH FTWHAKSKVE YKLGFQVDNV LAMDMPQVNI SVQGEVPRTL SVFRVELSCT GKVDSEVMIL MQLNLTVNSS KNFTVLNFKR RKMCYKKLEE VKTSALDKNT SRTIYDPVHA APTTSTR
预测分子量	49 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇与RYK重组蛋白相关的参考文献示例(注：文献为模拟概括，实际引用请核实原文)：

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1. **文献名称**: *Structural insights into the RYK extracellular domain and its Wnt5a interaction*

**作者**: Smith J, et al.

**摘要**: 本研究通过重组表达RYK受体胞外域蛋白，解析了其晶体结构，揭示了其非典型Wnt结合区域的关键表位，并证明重组RYK蛋白与Wnt5a的相互作用在轴突导向中具有调控作用。

2. **文献名称**: *RYK functions as a coreceptor in BMP signaling via recombinant domain mapping*

**作者**: Lee H, Green MJ.

**摘要**: 通过构建RYK重组蛋白的胞内域突变体，发现其与BMP信号通路中Smad蛋白的相互作用，揭示了RYK在胚胎干细胞分化中作为BMP辅助受体的新机制。

3. **文献名称**: *Recombinant RYK-Fc fusion protein inhibits tumor progression in glioblastoma models*

**作者**: Chen X, et al.

**摘要**: 利用RYK胞外段重组融合蛋白(RYK-Fc)阻断Wnt-RYK信号传导，在胶质母细胞瘤模型中显著抑制肿瘤侵袭性生长，提示其作为潜在治疗靶点的价值。

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如需具体文献，建议在PubMed或Google Scholar中检索关键词“RYK recombinant protein”或结合研究领域(如神经发育、癌症)进一步筛选。

背景信息

**Background of RYK Recombinant Protein**

The RYK (Related to receptor tyrosine kinase) protein, a member of the receptor tyrosine kinase (RTK) family, is a unique cell-surface receptor lacking intrinsic kinase activity due to evolutionary modifications in its catalytic domain. Initially identified for its role in developmental processes, RYK functions as a co-receptor in non-canonical Wnt signaling pathways, particularly interacting with Wnt5a to regulate cell polarity, migration, and tissue patterning. It is critical in embryogenesis, neural development, and stem cell differentiation, with notable involvement in axon guidance, skeletal morphogenesis, and organogenesis.

Structurally, RYK features an extracellular leucine-rich repeat (LRR) domain for ligand binding, a transmembrane domain, and an intracellular pseudokinase domain that recruits downstream signaling partners like Dishevelled (DVL) and Src family kinases. Its atypical signaling mechanism relies on proteolytic cleavage or interaction with other receptors (e.g., Frizzled) to activate pathways such as planar cell polarity (PCP) and calcium signaling.

RYK recombinant proteins, produced via heterologous expression systems (e.g., mammalian or bacterial cells), are engineered to study its biological functions and therapeutic potential. These proteins retain key domains for ligand binding and signaling, enabling in vitro and in vivo exploration of RYK’s role in diseases. Dysregulation of RYK is linked to cancers, neurodegenerative disorders (e.g., Alzheimer’s), and congenital defects, making it a target for drug development. Recombinant RYK tools aid in deciphering its crosstalk with Wnt pathways, screening inhibitors, or developing biologics to modulate its activity. Ongoing research aims to harness RYK’s regulatory mechanisms for regenerative medicine and precision therapies.