| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADCY7 |
| Uniprot No | P51828 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1080aa |
| 氨基酸序列 | MPAKGRYFLNEGEEGPDQDALYEKYQLTSQHGPLLLTLLLVAATACVALIIIAFSQGDPSRHQAILGMAFLVLAVFAALSVLMYVECLLRRWLRALALLTWACLVALGYVLVFDAWTKAACAWEQVPFFLFIVFVVYTLLPFSMRGAVAVGAVSTASHLLVLGSLMGGFTTPSVRVGLQLLANAVIFLCGNLTGAFHKHQMQDASRDLFTYTVKCIQIRRKLRIEKRQQENLLLSVLPAHISMGMKLAIIERLKEHGDRRCMPDNNFHSLYVKRHQNVSILYADIVGFTQLASDCSPKELVVVLNELFGKFDQIAKANECMRIKILGDCYYCVSGLPVSLPTHARNCVKMGLDMCQAIKQVREATGVDINMRVGIHSGNVLCGVIGLRKWQYDVWSHDVSLANRMEAAGVPGRVHITEATLKHLDKAYEVEDGHGQQRDPYLKEMNIRTYLVIDPRSQQPPPPSQHLPRPKGDAALKMRASVRMTRYLESWGAARPFAHLNHRESVSSGETHVPNGRRPKSVPQRHRRTPDRSMSPKGRSEDDSYDDEMLSAIEGLSSTRPCCSKSDDFYTFGSIFLEKGFEREYRLAPIPRARHDFACASLIFVCILLVHVLLMPRTAALGVSFGLVACVLGLVLGLCFATKFSRCCPARGTLCTISERVETQPLLRLTLAVLTIGSLLTVAIINLPLMPFQVPELPVGNETGLLAASSKTRALCEPLPYYTCSCVLGFIACSVFLRMSLEPKVVLLTVALVAYLVLFNLSPCWQWDCCGQGLGNLTKPNGTTSGTPSCSWKDLKTMTNFYLVLFYITLLTLSRQIDYYCRLDCLWKKKFKKEHEEFETMENVNRLLLENVLPAHVAAHFIGDKLNEDWYHQSYDCVCVMFASVPDFKVFYTECDVNKEGLECLRLLNEIIADFDELLLKPKFSGVEKIKTIGSTYMAAAGLSVASGHENQELERQHAHIGVMVEFSIALMSKLDGINRHSFNSFRLRVGINHGPVIAGVIGARKPQYDIWGNTVNVASRMESTGELGKIQVTEETCTILQGLGYSCECRGLINVKGKGELRTYFVCTDTAKFQGLGLN |
| 预测分子量 | 120,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADCY7重组蛋白的模拟参考文献示例(内容为虚构，仅供格式参考)：
1. **标题**：*"Recombinant ADCY7 Expression and cAMP Signaling in Macrophages"*
**作者**：Smith J, et al.
**摘要**：研究通过大肠杆菌系统表达重组ADCY7蛋白，分析其在巨噬细胞中的cAMP生成功能，发现ADCY7通过TLR4通路调控炎症反应。
2. **标题**：*"Structural Characterization of Human ADCY7 Catalytic Domain"*
**作者**：Lee H, et al.
**摘要**：利用杆状病毒-昆虫细胞系统表达ADCY7重组催化结构域，结合X射线晶体学解析其三维结构，揭示与G蛋白偶联的活性位点特征。
3. **标题**：*"ADCY7 Knockout and Recombinant Rescue in Hematopoietic Cells"*
**作者**：Garcia R, et al.
**摘要**：通过CRISPR构建ADCY7敲除细胞系，并转染重组ADCY7验证其对B淋巴细胞分化的必要性，证实其介导的cAMP-PKA通路调控作用。
4. **标题**：*"High-Throughput Screening of ADCY7 Inhibitors Using Purified Recombinant Protein"*
**作者**：Wang Y, et al.
**摘要**：优化ADCY7重组蛋白的哺乳动物细胞表达与纯化工艺，建立体外酶活检测体系，筛选获得新型小分子抑制剂，潜在应用于白血病治疗。
注：以上文献为示例，实际研究中请通过PubMed、Google Scholar等平台检索真实文献。
Adenylyl cyclase 7 (ADCY7) is a member of the membrane-bound adenylyl cyclase family, enzymes that catalyze the conversion of ATP to cyclic AMP (cAMP), a critical secondary messenger in cellular signaling. ADCY7 is encoded by the ADCY7 gene and plays a role in regulating physiological processes such as immune responses, neuronal signaling, and metabolic homeostasis. Unlike other isoforms, ADCY7 is primarily regulated by G-protein-coupled receptors (GPCRs) through interactions with Gαs and Gβγ subunits, linking extracellular signals to intracellular cAMP production. Its activity is also modulated by calcium and protein kinases, reflecting its integration into complex signaling networks.
Recombinant ADCY7 protein is produced using expression systems (e.g., bacterial, insect, or mammalian cells) to enable functional and structural studies. Purification typically involves affinity tags (e.g., His-tag) followed by chromatography, ensuring high purity and enzymatic activity. This recombinant form retains the catalytic core and regulatory domains, allowing researchers to investigate its activation mechanisms, inhibitor screening, and interactions with signaling partners. Structurally, ADCY7 contains two transmembrane domains anchoring it to the membrane and two cytoplasmic catalytic domains responsible for ATP cyclization.
Research on recombinant ADCY7 has implications for understanding diseases linked to cAMP dysregulation, including immune disorders, neurodegenerative conditions, and cancer. For example, ADCY7 is implicated in leukocyte function and inflammation, making it a potential therapeutic target. Additionally, its role in brain regions like the hippocampus highlights relevance in cognitive processes. By studying recombinant ADCY7. scientists aim to unravel isoform-specific regulatory pathways and develop targeted therapies modulating cAMP-dependent signaling with precision.
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