| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NDUFB7 |
| Uniprot No | P17568 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-137aa |
| 氨基酸序列 | GAHLVRRYLGDASVEPDPLQMPTFPPDYGFPERKEREMVATQQEMMDAQLRLQLRDYCAHHLIRLLKCKRDSFPNFLACKQERHDWDYCEHRDYVMRMKEFERERRLLQRKKRREKKAAELAKGQGPGEVDPKVAL |
| 预测分子量 | 43.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NDUFB7重组蛋白的参考文献示例(注：以下内容为示例性虚构，实际文献需通过学术数据库检索确认)：
1. **文献名称**：*Role of NDUFB7 in Mitochondrial Complex I Assembly: Insights from Recombinant Protein Expression*
**作者**：Smith A, et al.
**摘要**：本研究通过在大肠杆菌中表达并纯化重组NDUFFB7蛋白，揭示了其在线粒体复合体I组装中的关键作用，证明其缺失会导致复合体稳定性下降及呼吸链功能障碍。
2. **文献名称**：*NDUFB7 Recombinant Protein Restores Oxidative Phosphorylation in Cellular Models of Mitochondrial Disease*
**作者**：Lee J, et al.
**摘要**：利用哺乳动物细胞系统表达NDUFB7重组蛋白，发现其可挽救由NDUFB7基因突变引起的细胞氧化磷酸化缺陷，为线粒体疾病治疗提供潜在策略。
3. **文献名称**：*Structural and Functional Analysis of NDUFB7 Using Cryo-EM and Recombinant Protein Techniques*
**作者**：Zhang Y, et al.
**摘要**：通过冷冻电镜技术结合重组NDUFB7蛋白的功能实验，解析了其在复合体I中的精确定位及对电子传递链活性的调控机制。
4. **文献名称**：*NDUFB7 Overexpression via Recombinant Lentivirus Enhances Mitochondrial Metabolism in Cancer Cells*
**作者**：Johnson R, et al.
**摘要**：构建NDUFB7重组慢病毒载体并在肿瘤细胞中过表达，发现其通过增强复合体I活性促进ATP生成，影响肿瘤代谢重编程过程。
建议通过PubMed或Web of Science以“NDUFB7 recombinant”为关键词检索最新文献以获取真实数据。
NDUFB7 (NADH:ubiquinone oxidoreductase subunit B7) is a nuclear-encoded component of mitochondrial Complex I, the largest enzyme in the electron transport chain responsible for NADH oxidation and proton pumping. As part of the membrane arm of Complex I, NDUFB7 contributes to structural stability and catalytic activity. Its role is critical for cellular energy production, redox balance, and mitochondrial metabolism.
Recombinant NDUFB7 protein is engineered for in vitro studies to investigate its molecular interactions, structural dynamics, and functional defects linked to diseases. Mutations in NDUFB7 are associated with mitochondrial disorders, such as Leigh syndrome and cardiomyopathy, as well as cancer progression due to impaired oxidative phosphorylation. The recombinant form allows researchers to analyze pathogenic variants, map binding interfaces with other Complex I subunits (e.g., NDUFB6. NDUFB8), and screen therapeutic compounds targeting mitochondrial dysfunction.
Produced using expression systems like *E. coli* or mammalian cells, recombinant NDUFB7 is often tagged for purification and detection. Studies using this protein have advanced our understanding of Complex I assembly mechanisms and its role in metabolic reprogramming. Additionally, it serves as a tool for developing diagnostic assays and personalized treatments for mitochondrial diseases. Despite progress, challenges remain in mimicking native post-translational modifications, highlighting the need for advanced recombinant expression platforms. Research on NDUFB7 continues to bridge gaps in mitochondrial biology and precision medicine.
×
