Recombinant Human ADCY8 protein

中文名： 腺苷酸环化酶8(ADCY8)重组蛋白
别名： ADCY8；Adenylate cyclase type 8

货号: PA1000-9088

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	ADCY8
Uniprot No	P40145
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-1251aa
氨基酸序列	MELSDVRCLTGSEELYTIHPTPPAGDGRSASRPQRLLWQTAVRHITEQRFIHGHRGGSGSGSGGSGKASDPAGGGPNHHAPQLSGDSALPLYSLGPGERAHSTCGTKVFPERSGSGSASGSGGGGDLGFLHLDCAPSNSDFFLNGGYSYRGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSLASAPMDPLKGILLGFFTGIEVVICALVVVRKDTTSHTYLQYSGVVTWVAMTTQILAAGLGYGLLGDGIGYVLFTLFATYSMLPLPLTWAILAGLGTSLLQVILQVVIPRLAVISINQVVAQAVLFMCMNTAGIFISYLSDRAQRQAFLETRRCVEARLRLETENQRQERLVLSVLPRFVVLEMINDMTNVEDEHLQHQFHRIYIHRYENVSILFADVKGFTNLSTTLSAQELVRMLNELFARFDRLAHEHHCLRIKILGDCYYCVSGLPEPRQDHAHCCVEMGLSMIKTIRYVRSRTKHDVDMRIGIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLNGDYNVEEGHGKERNEFLRKHNIETYLIKQPEDSLLSLPEDIVKESVSSSDRRNSGATFTEGSWSPELPFDNIVGKQNTLAALTRNSINLLPNHLAQALHVQSGPEEINKRIEHTIDLRSGDKLRREHIKPFSLMFKDSSLEHKYSQMRDEVFKSNLVCAFIVLLFITAIQSLLPSSRVMPMTIQFSILIMLHSALVLITTAEDYKCLPLILRKTCCWINETYLARNVIIFASILINFLGAILNILWCDFDKSIPLKNLTFNSSAVFTDICSYPEYFVFTGVLAMVTCAVFLRLNSVLKLAVLLIMIAIYALLTETVYAGLFLRYDNLNHSGEDFLGTKEVSLLLMAMFLLAVFYHGQQLEYTARLDFLWRVQAKEEINEMKELREHNENMLRNILPSHVARHFLEKDRDNEELYSQSYDAVGVMFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELLGEDRFQDIEKIKTIGSTYMAVSGLSPEKQQCEDKWGHLCALADFSLALTESIQEINKHSFNNFELRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVPEETYLILKDQGFAFDYRGEIYVKGISEQEGKIKTYFLLGRVQPNPFILPPRRLPGQYSLAAVVLGLVQSLNRQRQKQLLNENNNTGIIKGHYNRRTLLSPSGTEPGAQAEGTDKSDLP
预测分子量	140 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于ADCY8重组蛋白的参考文献示例(注：以下文献信息为模拟虚构，仅供参考)：

1. **标题**：Recombinant Human ADCY8 Expression and Characterization in HEK293 Cells

**作者**：Smith A, et al.

**摘要**：研究报道了在HEK293细胞中高效表达重组人ADCY8蛋白的方法，并证实其钙离子依赖性腺苷酸环化酶活性，为体外研究ADCY8信号通路提供了工具。

2. **标题**：Structural Insights into ADCY8 Activation by G-Protein Coupling

**作者**：Chen L, et al.

**摘要**：通过冷冻电镜解析ADCY8重组蛋白与Gs蛋白复合物的三维结构，揭示了ADCY8被Gα亚基激活的分子机制，解释了其cAMP合成的变构调控。

3. **标题**：ADCY8 Recombinant Protein Rescue in Neuronal cAMP Deficiency Models

**作者**：Wang X, et al.

**摘要**：利用重组ADCY8蛋白在小鼠神经元模型中恢复cAMP水平，证明ADCY8功能缺陷与记忆障碍相关，提示其作为治疗靶点的潜力。

4. **标题**：Calmodulin Interaction with ADCY8: A Recombinant Protein-Based Study

**作者**：Kimura T, et al.

**摘要**：通过重组ADCY8蛋白体外结合实验，发现钙调蛋白(Calmodulin)在钙离子存在时增强ADCY8酶活性，阐明钙信号与cAMP通路交叉调控机制。

(提示：实际文献需通过PubMed等学术数据库检索，可使用关键词“ADCY8 recombinant”“adenylyl cyclase 8 expression”进一步筛选。)

背景信息

Adenylyl cyclase 8 (ADCY8) is a membrane-bound enzyme belonging to the adenylate cyclase family, which catalyzes the conversion of ATP to cyclic AMP (cAMP), a critical second messenger in cellular signaling. As a recombinant protein, ADCY8 is typically engineered for in vitro studies to explore its structure, function, and regulatory mechanisms. The ADCY8 gene encodes a calcium/calmodulin-sensitive isoform predominantly expressed in the brain, heart, and endocrine tissues, where it integrates extracellular signals through G protein-coupled receptors (GPCRs) and calcium signaling pathways.

Recombinant ADCY8 is often produced in heterologous expression systems like HEK293 or insect cells, tagged with purification markers (e.g., His-tag) for efficient isolation. Its structure includes two cytoplasmic domains (C1 and C2) that form the catalytic core, along with transmembrane domains anchoring it to the plasma membrane. Studies highlight its unique activation by calcium/calmodulin, distinguishing it from other cAMP-producing isoforms. This property links ADCY8 to calcium-mediated processes such as neurotransmitter release, hormone secretion, and memory formation.

Dysregulation of ADCY8 has been implicated in neurological disorders, cancer, and cardiovascular diseases, making its recombinant form a valuable tool for drug discovery and mechanistic studies. Researchers use it to screen modulators targeting cAMP pathways or to model diseases caused by ADCY8 mutations. Additionally, its role in insulin secretion and cardiac function underscores its therapeutic potential. Recombinant ADCY8 enables precise analysis of enzyme kinetics, post-translational modifications, and interactions with regulatory proteins, advancing our understanding of cAMP-dependent signaling networks in health and disease.