| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF549 |
| Uniprot No | Q6P9A3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-640 aa |
| 活性数据 | MAEAALVNTPQIPMVTEEFVKPSQGHVTFEDIAVYFSQEEWGLLDEAQRCLYHDVMLENFSLMASVGCLHGIEAEEAPSEQTLSAQGVSQARTPKLGPSIPNAHSCEMCILVMKDILYLSEHQGTLPWQKPYTSVASGKWFSFGSNLQQHQNQDSGEKHIRKEESSALLLNSCKIPLSDNLFPCKDVEKDFPTILGLLQHQTTHSRQEYAHRSRETFQQRRYKCEQVFNEKVHVTEHQRVHTGEKAYKRREYGKSLNSKYLFVEHQRTHNAEKPYVCNICGKSFLHKQTLVGHQQRIHTRERSYVCIECGKSLSSKYSLVEHQRTHNGEKPYVCNVCGKSFRHKQTFVGHQQRIHTGERPYVCMECGKSFIHSYDRIRHQRVHTGEGAYQCSECGKSFIYKQSLLDHHRIHTGERPYECKECGKAFIHKKRLLEHQRIHTGEKPYVCIICGKSFIRSSDYMRHQRIHTGERAYECSDCGKAFISKQTLLKHHKIHTRERPYECSECGKGFYLEVKLLQHQRIHTREQLCECNECGKVFSHQKRLLEHQKVHTGEKPCECSECGKCFRHRTSLIQHQKVHSGERPYNCTACEKAFIYKNKLVEHQRIHTGEKPYECGKCGKAFNKRYSLVRHQKVHITEEP |
| 分子量 | 100.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF549蛋白的模拟参考文献示例(注：部分文献信息为假设性示例，实际引用时请核实真实文献)：
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1. **文献名称**: *Cloning and Functional Characterization of Human ZNF549 Gene*
**作者**: Li, X., et al.
**摘要**: 本研究报道了人ZNF549基因的克隆及其在大肠杆菌中的重组表达。通过生物信息学分析显示，ZNF549蛋白包含典型的C2H2型锌指结构域。实验验证其具备DNA结合活性，可能在转录调控中发挥作用。
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2. **文献名称**: *ZNF549 Promotes Tumor Progression via Epigenetic Silencing in Gastric Cancer*
**作者**: Wang, Y., et al.
**摘要**: 研究者利用重组ZNF549蛋白进行体外实验，发现其通过招募组蛋白去乙酰化酶(HDACs)抑制靶基因表达，促进胃癌细胞增殖和转移，提示ZNF549可能作为癌症治疗的潜在靶点。
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3. **文献名称**: *Structural Analysis of Recombinant ZNF549 and Its Interaction with DNA Motifs*
**作者**: Zhang, R., et al.
**摘要**: 通过X射线晶体学解析了重组ZNF549蛋白的锌指结构域三维结构，并鉴定其特异性结合的DNA序列(如5’-GCCAGT-3’)，为研究其在基因调控中的作用机制提供结构基础。
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**注意**：以上文献为模拟生成，实际研究中建议通过PubMed、Google Scholar等平台检索真实文献(关键词：ZNF549. recombinant protein, zinc finger)，并确保引用准确性。
**Background of Recombinant Human ZNF549 Protein**
Zinc finger protein 549 (ZNF549), a member of the Krüppel-associated box (KRAB)-containing zinc finger protein (ZNF) family, is encoded by the *ZNF549* gene located on human chromosome 19. ZNF proteins are characterized by tandem Cys2-His2 (C2H2) zinc finger domains, which facilitate sequence-specific DNA binding, and many act as transcription regulators. ZNF549 contains multiple zinc finger motifs, suggesting its potential role in nucleic acid interaction and gene expression modulation. While functional studies on ZNF549 remain limited, its structural homology to other KRAB-ZNFs implies involvement in chromatin remodeling, transcriptional repression, or regulation of developmental processes.
Recombinant human ZNF549 protein is engineered for in vitro studies, typically expressed in heterologous systems (e.g., *E. coli* or mammalian cells) with tags for purification. Its production enables biochemical and molecular investigations, such as identifying DNA-binding motifs, mapping protein-protein interactions, or exploring its role in signaling pathways. Interest in ZNF549 also stems from its potential links to diseases; dysregulation of ZNF family members has been associated with cancers, neurological disorders, and immune dysfunction. However, specific mechanistic insights into ZNF549’s physiological or pathological relevance await further exploration.
Current research focuses on elucidating its target genes, regulatory networks, and possible therapeutic applications, leveraging recombinant protein tools to bridge gaps in understanding this understudied transcriptional regulator.
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