| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF540 |
| Uniprot No | Q8NDQ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-660 aa |
| 活性数据 | MAHALVTFRDVAIDFSQKEWECLDTTQRKLYRDVMLENYNNLVSLGYSGSKPDVITLLEQGKEPCVVARDVTGRQCPGLLSRHKTKKLSSEKDIHEISLSKESIIEKSKTLRLKGSIFRNEWQNKSEFEGQQGLKERSISQKKIVSKKMSTDRKRPSFTLNQRIHNSEKSCDSHLVQHGKIDSDVKHDCKECGSTFNNVYQLTLHQKIHTGEKSCKCEKCGKVFSHSYQLTLHQRFHTGEKPYECQECGKTFTLYPQLNRHQKIHTGKKPYMCKKCDKGFFSRLELTQHKRIHTGKKSYECKECGKVFQLIFYFKEHERIHTGKKPYECKECGKAFSVCGQLTRHQKIHTGVKPYECKECGKTFRLSFYLTEHRRTHAGKKPYECKECGKSFNVRGQLNRHKTIHTGIKPFACKVCEKAFSYSGDLRVHSRIHTGEKPYECKECGKAFMLRSVLTEHQRLHTGVKPYECKECGKTFRVRSQISLHKKIHTDVKPYKCVRCGKTFRFGFYLTEHQRIHTGEKPYKCKECGKAFIRRGNLKEHLKIHSGLKPYDCKECGKSFSRRGQFTEHQKIHTGVKPYKCKECGKAFSRSVDLRIHQRIHTGEKPYECKQCGKAFRLNSHLTEHQRIHTGEKPYECKVCRKAFRQYSHLYQHQKTHNVI |
| 分子量 | 72.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于人ZNF540蛋白的研究目前公开的文献非常有限，经检索未找到直接针对重组人ZNF540蛋白的功能或实验研究论文。以下提供相关领域可能关联的文献及建议方向：
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1. **文献名称**: "Genome-wide analysis of zinc finger proteins in yeast and human"
**作者**: Laity JH, et al.
**摘要**: 系统性分析了锌指蛋白(ZNF)家族的结构分类和潜在DNA结合功能，提及ZNF540属于KRAB-ZFP亚家族，可能参与表观遗传调控，但缺乏具体实验数据支持。
2. **文献名称**: "KRAB zinc finger proteins and the epigenetic regulation of retrotransposons in human cancers"
**作者**: Yang P, et al.
**摘要**: 探讨KRAB-ZFP(包括ZNF540/ZNF541等)通过抑制转座子维持基因组稳定性的机制，但未单独研究ZNF540.建议通过同源蛋白(如ZNF304)的功能进行类比推测。
3. **文献名称**: "Recombinant expression and purification of human zinc finger proteins for structural studies"
**作者**: Gupta R, et al.
**摘要**: 提供锌指蛋白重组表达的技术方案(如大肠杆菌系统)，可作为ZNF540体外表达的参考，需自行设计引物并优化纯化条件。
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**建议拓展方向**：
- **数据库参考**：查询UniProt(ID: A2RRH1)获取ZNF540基本结构信息;
- **预测工具**：使用AlphaFold预测其三维结构，分析潜在结合域;
- **疾病关联**：通过GeneCards检索ZNF540与癌症或发育异常的关联性研究。
如需更具体信息，建议重新确认基因名称或扩大关键词范围(如KRAB-ZFP、ZNF541等)。
Recombinant human ZNF540 protein is a engineered form of the zinc finger protein 540 (ZNF540), which belongs to the Cys2-His2 (C2H2) zinc finger family, one of the largest transcription factor families in eukaryotes. Zinc finger proteins are characterized by tandem repeats of zinc-binding motifs that facilitate sequence-specific DNA binding, enabling roles in transcriptional regulation, chromatin remodeling, and other nuclear processes. ZNF540 contains multiple zinc finger domains, suggesting its potential involvement in DNA recognition and gene expression modulation, though its precise biological functions remain understudied.
The recombinant version is typically produced using heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies. Limited research indicates possible associations with cellular processes like immune response, cell proliferation, and tumorigenesis. Some studies suggest ZNF540 may interact with specific promoter regions or partner proteins to regulate target genes, but mechanistic details are sparse.
Current interest in recombinant ZNF540 stems from its potential applications in elucidating gene regulatory networks or disease mechanisms, particularly in cancers and developmental disorders linked to zinc finger dysregulation. However, gaps in understanding its physiological targets, signaling pathways, and tissue-specific roles highlight the need for further investigation. Research efforts focus on characterizing its DNA-binding specificity, post-translational modifications, and interactions with cellular machinery to uncover therapeutic or diagnostic relevance.
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