| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF547 |
| Uniprot No | Q8IVP9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-402 aa |
| 活性数据 | MAEMNPAQGHVVFEDVAIYFSQEEWGHLDEAQRLLYRDVMLENLALLSSLGCCHGAEDEEAPLEPGVSVGVSQVMAPKPCLSTQNTQPCETCSSLLKDILRLAEHDGTHPEQGLYTCPAHLHQHQKEQIREKLSRGDGGRPTFVKNHRVHMAGKTFLCSECGKAFSHKHKLSDHQKIHTGERTYKCSKCGILFMERSTLNRHQRTHTGERPYECNECGKAFLCKSHLVRHQTIHSGERPYECSECGKLFMWSSTLITHQRVHTGKRPYGCSECGKFFKCNSNLFRHYRIHTGKRSYGCSECGKFFMERSTLSRHQRVHTGERPYECNECGKFFSLKSVLIQHQRVHTGERPYECSECGKAFLTKSHLICHQTVHTAAKQCSECGKFFRYNSTLLRHQKVHTG |
| 分子量 | 72.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人ZNF547蛋白研究的相关文献示例(注意：实际文献可能存在研究局限性，此处为模拟内容，建议通过学术数据库核实)：
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1. **"Characterization of the Zinc Finger Protein ZNF547 and Its Role in Transcriptional Regulation"**
**作者**: Tanaka, R. et al.
**摘要**: 本研究首次报道了重组人ZNF547蛋白的体外表达与纯化，通过ChIP-seq分析发现ZNF547结合在多个基因启动子区域，可能参与调控细胞分化相关通路，尤其在神经发育中发挥作用。
2. **"ZNF547 interacts with HDAC1 to repress oncogenic signaling in colorectal cancer"**
**作者**: Chen, L. & Wang, H.
**摘要**: 该文献揭示ZNF547与组蛋白去乙酰化酶HDAC1形成复合物，通过抑制Wnt/β-catenin信号通路抑制结直肠癌细胞增殖，重组ZNF547蛋白的过表达在体外实验中被证明可诱导肿瘤细胞凋亡。
3. **"Structural basis of DNA recognition by the human zinc finger protein ZNF547"**
**作者**: Park, S. et al.
**摘要**: 通过X射线晶体学解析了ZNF547蛋白锌指结构域的DNA结合模式，发现其特异性识别GC-rich序列，为设计针对ZNF547功能的小分子抑制剂提供了结构基础。
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**说明**：
ZNF547作为锌指蛋白家族成员，现有研究多聚焦于其基因调控功能及疾病关联，但针对重组蛋白的深入功能研究仍较少。建议通过PubMed或Web of Science以“ZNF547 recombinant protein”为关键词检索最新文献。
Zinc finger protein 547 (ZNF547), a member of the C2H2-type zinc finger protein family, is encoded by the ZNF547 gene located on human chromosome 19. Zinc finger proteins are characterized by tandem repeats of zinc-binding motifs that facilitate sequence-specific interactions with DNA, RNA, or proteins. ZNF547 contains multiple Krüppel-associated box (KRAB) domains and C2H2 zinc finger motifs, suggesting roles in transcriptional regulation and chromatin remodeling. As a transcription factor, it likely participates in the modulation of gene expression by binding to promoter or enhancer regions, though its exact physiological targets and regulatory mechanisms remain understudied.
Recombinant human ZNF547 protein is typically produced in vitro using heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Its recombinant form allows researchers to investigate DNA-binding specificity, protein-protein interactions, and potential involvement in cellular processes like differentiation, apoptosis, or stress response. Emerging evidence links ZNF family proteins to diseases such as cancer and neurodegenerative disorders, positioning ZNF547 as a candidate for exploring disease mechanisms. However, detailed biological functions of ZNF547. including its tissue-specific roles or post-translational modifications, require further characterization. Current research focuses on unraveling its molecular interactions and possible therapeutic relevance.
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