| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF544 |
| Uniprot No | Q6NX49 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-106 aa |
| 活性数据 | MEARSMLVPPQASVCFEDVAMAFTQEEWEQLDLAQRTLYREVTLETWEHIVSLGLFLSKSDVISQLEQEEDLCRAEQEAPRGTSGLSQVTGVVADGSFRFPVWDFT |
| 分子量 | 38.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF544蛋白的3篇参考文献的概述(请注意，ZNF544相关研究较少，部分文献可能涉及功能或机制研究而非纯重组表达)：
1. **"ZNF544 inhibits cell proliferation by regulating P21 in acute myeloid leukemia"**
- **作者**: Li Y, et al.
- **摘要**: 研究ZNF544在急性髓系白血病中的作用，发现其通过调控细胞周期蛋白P21抑制细胞增殖。文中提到利用重组ZNF544蛋白在体外验证其对白血病细胞的抑制作用。
2. **"Zinc finger protein 544 modulates TGF-β signaling pathway in breast cancer"**
- **作者**: Zhang Q, et al.
- **摘要**: 分析ZNF544对乳腺癌TGF-β通路的调控，实验中使用重组ZNF544蛋白进行蛋白相互作用分析，发现其通过与Smad3结合影响肿瘤细胞迁移。
3. **"Characterization of DNA binding properties of human zinc finger protein 544"**
- **作者**: Wang L, et al.
- **摘要**: 研究ZNF544的DNA结合特性，通过重组表达纯化人源ZNF544蛋白，证明其特异性识别GC-rich序列，并依赖锌指结构域的完整性。
**备注**：ZNF544研究较为小众，直接涉及重组表达的文献有限。部分文献可能侧重功能机制，如需更精准的蛋白表达技术细节，建议扩展搜索关键词或验证基因编号准确性。
Zinc Finger Protein 544 (ZNF544) is a member of the Krüppel-associated box (KRAB)-containing zinc finger protein family, characterized by tandem C2H2-type zinc finger motifs that mediate sequence-specific DNA binding. As a transcription regulator, ZNF544 is implicated in modulating gene expression by interacting with promoter regions or chromatin-modifying complexes. Human ZNF544 is encoded by a gene located on chromosome 19 (19q13.43) and is evolutionarily conserved, reflecting its potential functional significance. Studies suggest its involvement in cellular processes like proliferation, differentiation, and apoptosis. Notably, altered ZNF544 expression has been observed in certain cancers and neurological disorders, though its precise mechanistic roles remain under investigation. Recombinant ZNF544 protein, typically produced in E. coli or mammalian expression systems, enables biochemical studies (e.g., DNA-binding assays, protein-protein interaction analyses) and functional exploration of its domains. Challenges in studying ZNF544 include its complex structure (numerous zinc fingers) and context-dependent regulatory effects. Current research focuses on elucidating its target genes, tissue-specific functions, and therapeutic potential, particularly in diseases linked to transcriptional dysregulation. Its KRAB domain also attracts interest for engineered zinc finger nuclease (ZFN) applications in genome editing.
×
