| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF543 |
| Uniprot No | Q08ER8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-600 aa |
| 活性数据 | MAASAQVSVTFEDVAVTFTQEEWGQLDAAQRTLYQEVMLETCGLLMSLGCPLFKPELIYQLDHRQELWMATKDLSQSSYPGDNTKPKTTEPTFSHLALPEEVLLQERLTQGASKNSQLGQSKDQDGPSEMQEVHLKIGIGPQRGKLLEKMSSERDGLGSDDGVCTKITQKQVSTEGDLYECDSHGPVTDALIREEKNSYKCEECGKVFKKNALLVQHERIHTQVKPYECTECGKTFSKSTHLLQHHIIHTGEKPYKCMECGKAFNRRSHLTRHQRIHSGEKPYKCSVCGKAFTHRSTFVLHHRSHTGEKPFVCKECGKAFRDRPGFIRHYIIHTGEKPYECIECGKAFNRRSYLTWHQQIHTGVKPFECNECGKAFCESADLIQHYIIHTGEKPYKCMECGKAFNRRSHLKQHQRIHTGEKPYECSECGKAFTHCSTFVLHKRTHTGEKPYECKECGKAFSDRADLIRHFSIHTGEKPYECVECGKAFNRSSHLTRHQQIHTGEKPYECIQCGKAFCRSANLIRHSIIHTGEKPYECSECGKAFNRGSSLTHHQRIHTGRNPTIVTDVGRPFMTAQTSVNIQELLLGKEFLNITTEENLW |
| 分子量 | 94.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF543蛋白的虚构参考文献示例，结构按文献名称、作者、摘要内容列出：
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### 1. **《重组人ZNF543蛋白的克隆表达及其DNA结合特性研究》**
**作者：Zhang L, Chen Y et al.**
**摘要**：本研究通过大肠杆菌系统成功克隆并表达重组人ZNF543蛋白，利用镍柱亲和层析纯化后，通过电泳迁移实验(EMSA)证实其特异性结合靶基因启动子区域的GT-box序列，提示ZNF543可能在转录调控中发挥重要作用。
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### 2. **《ZNF543的CRISPR/Cas9敲除及对肿瘤细胞增殖的影响》**
**作者：Wang X, Liu H et al.**
**摘要**：作者通过构建ZNF543重组蛋白的真核表达载体(HEK293细胞)，并结合CRISPR/Cas9敲除技术，发现ZNF543缺失导致肝癌细胞增殖抑制。蛋白质相互作用筛选实验进一步揭示其与Wnt/β-catenin通路蛋白的潜在关联。
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### 3. **《ZNF543的锌指结构域解析及其在神经分化中的功能》**
**作者：Guo R, Zhou T et al.**
**摘要**：通过X射线晶体学解析重组ZNF543蛋白的锌指结构域三维结构，揭示其C2H2型锌指模体特征。体外神经干细胞实验表明，ZNF543过表达显著促进分化标记物(如MAP2)的上调，暗示其在神经发育中的调控作用。
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### 4. **《ZNF543重组蛋白的高效表达及自身免疫性疾病中的抗体反应》**
**作者：Li J, Park S et al.**
**摘要**：优化毕赤酵母系统实现ZNF543的高效分泌表达，纯化蛋白用于ELISA筛查。发现系统性红斑狼疮(SLE)患者血清中抗ZNF543抗体水平显著升高，提示其可能作为自身免疫反应的潜在生物标志物。
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以上示例基于典型研究场景编写，未引用真实文献。如需实际文献，建议通过PubMed或Google Scholar搜索“ZNF543 recombinant protein”或“ZNF543 function”。
Zinc finger protein 543 (ZNF543) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, characterized by tandem C2H2-type zinc finger motifs that enable sequence-specific DNA binding. These proteins typically function as transcriptional regulators, interacting with chromatin-modifying complexes to repress or activate target genes. ZNF543 is encoded in humans on chromosome 19 (19q13.43) and is presumed to play roles in cellular differentiation, proliferation, and development, though its exact biological functions remain less characterized compared to other zinc finger proteins.
Studies suggest ZNF543 may participate in oncogenesis, with altered expression observed in certain cancers, potentially influencing tumor progression through gene regulatory networks. Its recombinant form, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables structural and functional studies, including DNA-protein interaction assays and exploration of its role in epigenetic regulation. Recent research highlights its interaction with retrotransposons and involvement in genome stability, aligning with KRAB-ZFPs' broader role in silencing transposable elements. As a recombinant tool, ZNF543 protein aids in dissecting mechanisms of gene silencing, offering insights for therapeutic strategies targeting transcriptional dysregulation. Further work is needed to map its precise targets and pathways in health and disease.
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