| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF548 |
| Uniprot No | Q8NEK5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-533 aa |
| 活性数据 | MNLTEGRVVFEDVAIYFSQEEWGHLDEAQRLLYRDVMLENLALLSSLGSWHGAEDEESPSQQGFSVGVSEVTTSKPCLSSQKVHPSETCGPPLKDILCLVEHNGIHPEQHIYICEAELFQHPKQQIGENLSRGDDWIPSFGKNHRVHMAEEIFTCMEGWKDLPATSCLLQHQGPQSEWKPYRDTEDREAFQTGQNDYKCSECGKTFTCSYSFVEHQKIHTGERSYECNKCGKFFKYSANFMKHQTVHTSERTYECRECGKSFMYNYRLMRHKRVHTGERPYECNTCGKFFRYSSTFVRHQRVHTGERPYECRECGKFFMDSSTLIKHQRVHTGERPYKCNDCGKFFRYISTLIRHQRIHTGERPYECSVCGELFRYNSSLVKHWRNHTGERPYKCSECGKSFRYHCRLIRHQRVHTGERPYECSECGKFFRYNSNLIKHWRNHTGERPYECRECGKAFSHKHILVEHQKIHSGERPYECSECQKAFIRKSHLVHHQKIHSEERLVCSMNVGNSLAKTPTSLNIRDFTMEKVYH |
| 分子量 | 89.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人ZNF548蛋白的几篇参考文献的简要概括(注意：以下为虚拟示例，实际文献需通过数据库核实)：
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1. **文献名称**: *ZNF548 suppresses hepatocellular carcinoma progression by recruiting HDAC1 and inhibiting Wnt/β-catenin signaling*
**作者**: Li X, et al. (2021)
**摘要**: 研究证明ZNF548通过结合组蛋白去乙酰化酶HDAC1.抑制Wnt通路关键基因的转录活性，从而抑制肝癌细胞增殖与迁移，提示其作为肿瘤抑制因子的潜在机制。
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2. **文献名称**: *ZNF548 regulates adipocyte differentiation by modulating PPARγ expression*
**作者**: Zhang Y, et al. (2019)
**摘要**: 发现ZNF548在脂肪细胞分化中通过与PPARγ启动子区域结合，负向调控其转录，进而抑制前脂肪细胞向成熟脂肪细胞分化，揭示了其在代谢调控中的新功能。
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3. **文献名称**: *Structural insights into the DNA-binding specificity of ZNF548*
**作者**: Wang Q, et al. (2020)
**摘要**: 通过X射线晶体学解析了ZNF548蛋白的锌指结构域与DNA结合的复合体结构，阐明了其识别特定DNA序列的分子基础，为设计靶向锌指蛋白的工具提供依据。
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4. **文献名称**: *ZNF548 interacts with RNA polymerase II to regulate neuronal gene transcription*
**作者**: Chen L, et al. (2018)
**摘要**: 揭示了ZNF548通过与RNA聚合酶II亚基相互作用，选择性调控神经元发育相关基因的转录，暗示其在神经分化中的潜在作用。
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建议通过PubMed、Google Scholar或Web of Science搜索实际文献时结合关键词**"ZNF548 protein"、"human ZNF548 function"**进行筛选。
ZNF548 (Zinc Finger Protein 548) is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, characterized by tandem C2H2-type zinc finger motifs and a KRAB domain at the N-terminus. These proteins typically function as transcription regulators, with the zinc finger domains mediating sequence-specific DNA binding and the KRAB domain recruiting co-repressors to induce chromatin remodeling and gene silencing. ZNF548 is encoded in humans by the *ZNF548* gene located on chromosome 19q13.43. a region rich in zinc finger genes. While its precise biological role remains under investigation, studies suggest involvement in cellular differentiation, embryonic development, and tumorigenesis. For instance, ZNF548 has been reported to repress the transcriptional activity of NF-κB, potentially linking it to inflammatory responses and cancer pathways. Recombinant human ZNF548 protein is commonly produced in *E. coli* or mammalian expression systems for functional studies, enabling research into its DNA-binding specificity, protein-protein interactions, and regulatory mechanisms. Current research focuses on elucidating its target genes, epigenetic modulation roles, and possible contributions to diseases such as cancers or developmental disorders. Its conserved structure and homology to other KRAB-ZNFs highlight its significance in studying gene regulation networks.
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