| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF545 |
| Uniprot No | Q6P9Y7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-532 aa |
| 活性数据 | MALRSVMFSDVSIDFSPEEWEYLDLEQKDLYRDVMLENYSNLVSLGCFISKPDVISSLEQGKEPWKVVRKGRRQYPDLETKYETKKLSLENDIYEINLSQWKIMERIENHGLKGLILKNDWESTGKIEGQERPQEGYFSSVKMPSEKVSSYQKRTSVTPHQRLHFVDKPYECKECGKAFRVRQQLTFHHRIHTGEKPYECKECGMAFRQTAHLTRHQRLHSGEKLYECKECGEAFICGADLRVHQKMHIGEKPYECKECGKAFRVRGQLTLHQRIHTGEKPYVCKECGKAFRQYAHLTRHQKLNSADRLYECKECGKAFLCGSGLRVHHKLHTGEKPYECKECGKAFRVRQQLTLHQRIHTGEKPYECKECGKTFSRGYHLILHHRIHTGEKPYECKECWKAFSRYSQLISHQSIHIGVKPYDCKECGKAFRLLSQLTQHQSIHIGEKPYKCKECGKAFRLRQKLTLHQSIHTGEKPFECKECRKAFRLNSSLIQHLRIHSGEKPYECKECKKAFRQHSHLTHHLKIHNVKI |
| 分子量 | 89 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF545蛋白的关键研究文献示例(以下内容为虚构演示，实际文献需查阅数据库如PubMed等)：
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1. **文献名称**: *ZNF545 functions as a tumor suppressor in hepatocellular carcinoma by regulating cell proliferation*
**作者**: Li X, Wang Y, Zhang H.
**摘要**: 本研究通过重组人ZNF545蛋白的体外表达，揭示了其通过抑制细胞周期蛋白(如Cyclin D1)和诱导凋亡来抑制肝癌细胞增殖的作用机制。
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2. **文献名称**: *Recombinant ZNF545 protein binds to DNA and modulates transcriptional repression in leukemia cells*
**作者**: Chen L, Liu R, Zhou M.
**摘要**: 利用大肠杆菌表达系统制备重组ZNF545蛋白，发现其在白血病细胞中通过特异性结合启动子区域的DNA序列，抑制促癌基因(如MYC)的转录活性。
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3. **文献名称**: *Structural analysis and functional characterization of recombinant human ZNF545 protein*
**作者**: Kim S, Park J, Lee K.
**摘要**: 首次解析了重组ZNF545蛋白的晶体结构，发现其锌指结构域对DNA结合至关重要，并通过体外实验验证了其在抑制乳腺癌细胞迁移中的功能。
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4. **文献名称**: *ZNF545 methylation and its role in cancer: Implications for recombinant protein-based therapy*
**作者**: Gupta A, Singh D.
**摘要**: 发现ZNF545在多种癌症中因启动子甲基化表达下调，重组ZNF545蛋白的体外递送可恢复肿瘤细胞对化疗药物的敏感性，提示其潜在治疗价值。
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**注意事项**：
- 以上文献为简化示例，真实文献需通过学术数据库检索;
- ZNF545的研究多集中于其抑癌功能及表观遗传调控，重组蛋白的应用常见于体外功能验证或结构研究中。
Zinc finger protein 545 (ZNF545), also known as ZFP82. is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein (KZNF) family, one of the largest transcription factor families in humans. These proteins are characterized by tandem C₂H₂-type zinc finger motifs that enable sequence-specific DNA binding, coupled with an N-terminal KRAB domain that typically mediates transcriptional repression through interactions with co-repressors like KAP1/TIF1β. ZNF545 is hypothesized to function as a tumor suppressor, with epigenetic silencing via promoter hypermethylation observed in multiple cancers, including hepatocellular carcinoma, colorectal cancer, and acute myeloid leukemia. Its downregulation has been associated with enhanced cell proliferation, migration, and apoptosis resistance.
The recombinant human ZNF545 protein, produced using expression systems like *E. coli* or mammalian cells, enables structural and functional studies to decipher its molecular mechanisms. Research focuses on its DNA-binding specificity, regulatory targets (e.g., cell cycle or apoptosis-related genes), and crosstalk with epigenetic modifiers. Its KRAB domain's role in recruiting chromatin-remodeling complexes to repress transposable elements or oncogenic pathways is of particular interest. Therapeutic exploration includes leveraging ZNF545’s tumor-suppressive potential via gene therapy or demethylation agents to reactivate its expression in malignancies. However, its full physiological roles and context-dependent regulatory networks remain under investigation, partly due to functional redundancy among KZNFs.
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