| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serpin B12 |
| Entrez GeneID | 89777; |
| WB Predicted band size | 46kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Serpin B12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Serpin B12抗体的3篇代表性文献的简要信息(基于公开研究数据虚构，仅供参考)：
1. **文献名称**: *"Serpin B12 regulates extracellular matrix remodeling via protease inhibition in keratinocytes"*
**作者**: Silverman GA, et al.
**摘要**: 研究揭示了Serpin B12通过抑制丝氨酸蛋白酶(如KLK5)调控表皮细胞分化和细胞外基质重塑，其抗体被用于定位其在皮肤组织中的表达模式。
2. **文献名称**: *"Characterization of a monoclonal antibody targeting Serpin B12 for functional studies in coagulation"*
**作者**: Kaiserman D, Bird PI.
**摘要**: 开发了一种特异性识别Serpin B12的单克隆抗体，证实其在凝血级联反应中对凝血酶样蛋白酶的抑制作用，并用于血浆样本中Serpin B12的定量检测。
3. **文献名称**: *"Serpin B12 as a potential biomarker in colorectal cancer: Immunohistochemical analysis using a novel antibody"*
**作者**: Zhou Q, et al.
**摘要**: 通过新型抗体检测发现，Serpin B12在结直肠癌组织中表达显著上调，提示其可能作为肿瘤进展的生物标志物或治疗靶点。
(注：以上文献为示例性内容，实际研究中请通过PubMed或Google Scholar检索真实文献。)
Serpin B12. a member of the serine protease inhibitor (serpin) superfamily, plays roles in regulating proteolytic pathways involved in coagulation, inflammation, and cellular homeostasis. It inhibits target proteases through a conformational change mechanism, forming stable complexes to block enzymatic activity. Located on chromosome 18q22 in humans, Serpin B12 shares structural homology with other intracellular serpins but exhibits distinct functional characteristics. Its precise physiological role remains less defined compared to well-studied serpins like antithrombin or α1-antitrypsin, though studies suggest potential involvement in skin barrier function, thrombosis, and immune response modulation.
Antibodies targeting Serpin B12 are critical tools for elucidating its expression, localization, and interactions. Polyclonal or monoclonal antibodies are typically generated using recombinant protein fragments or synthetic peptides, validated via techniques like Western blotting, immunohistochemistry, or ELISA. These antibodies aid in detecting Serpin B12 in tissues or cell lysates, revealing its expression patterns in epithelial cells, platelets, or specific immune cells. Challenges include ensuring specificity due to high sequence conservation among serpins. Research applications range from exploring its role in dermatological disorders (e.g., Netherton syndrome) to dissecting protease-regulated pathways in cancer or inflammation. Reliable Serpin B12 antibodies are essential for advancing mechanistic studies and therapeutic targeting.
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