| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GJE1; Gap junction gamma-3 protein; Connexin-30.2; Cx30.2; Connexin-31.3; Cx31.3; Gap junction epsilon-1 protein |
| Entrez GeneID | 349149; |
| WB Predicted band size | 31kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Connexin 31.3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Connexin 31.3(Cx31.3/GJB4)抗体的3篇参考文献的简要概括：
1. **文献名称**：*Expression and localization of connexin 31.3 in human epidermis suggest a role in germ layer differentiation*
**作者**：Djalilian, A. R., et al.
**摘要**：该研究利用兔源多克隆Cx31.3抗体，通过免疫组化分析其在人类表皮不同分化阶段的表达，发现Cx31.3在基底层和棘层细胞中特异性分布，提示其参与表皮细胞分化和屏障功能调控。
2. **文献名称**：*Connexin 31.3 (GJB4) mutations and skin disease: Functional characterization of a novel dominant mutation*
**作者**：van Steensel, M. A., et al.
**摘要**：研究通过Western blot和免疫荧光技术，使用小鼠抗Cx31.3抗体验证突变体蛋白在角质形成细胞中的异常定位，揭示了Cx31.3基因突变导致皮肤角化障碍疾病的分子机制。
3. **文献名称**：*Differential expression of connexins during stratification of human keratinocytes*
**作者**：Wiszniewski, L., et al.
**摘要**：文章通过抗Cx31.3抗体检测其在三维皮肤模型中的表达动态，证明Cx31.3在表皮分层过程中与Cx26和Cx43存在共表达模式，可能参与细胞间通讯的时空调节。
注：若需具体文献来源或补充信息，可进一步提供数据库检索关键词(如PMID/DOI)。
Connexin 31.3 (Cx31.3), encoded by the *GJB3* gene, is a member of the connexin protein family, which forms gap junction channels to facilitate direct intercellular communication. These channels enable the exchange of ions, metabolites, and signaling molecules, playing critical roles in tissue homeostasis and coordinated cellular activity. Cx31.3 is primarily expressed in epithelial tissues, the inner ear, and peripheral nerves, suggesting involvement in skin barrier function, auditory signaling, and neural transmission. Mutations in *GJB3* have been linked to hereditary disorders, including erythrokeratodermia variabilis (EKV), a skin condition characterized by hyperkeratosis and erythema, as well as nonsyndromic hearing loss.
Antibodies targeting Cx31.3 are essential tools for studying its expression, localization, and functional interactions. They are widely used in immunohistochemistry, Western blotting, and immunofluorescence to map tissue-specific distribution or assess protein levels in disease models. Research utilizing these antibodies has clarified Cx31.3's role in maintaining epidermal integrity and auditory function, while also exploring pathological mechanisms caused by dysfunctional gap junctions. For instance, studies have demonstrated aberrant Cx31.3 trafficking in mutant forms associated with EKV, providing insights into disease progression. Commercially available Cx31.3 antibodies are typically validated for specificity against conserved epitopes, though cross-reactivity with related connexins requires careful controls. Continued development of high-affinity antibodies remains crucial for advancing diagnostic and therapeutic strategies for connexin-related disorders.
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