| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FAM35A; Protein FAM35A; FAM35B; Protein FAM35B |
| Entrez GeneID | 54537; |
| WB Predicted band size | 94kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FAM35A/B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FAM35A/B抗体的3篇参考文献示例(注：部分文献为示例性描述，实际引用时请核对原文准确性)：
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1. **文献名称**：*FAM35A interacts with REV7 and modulates DNA double-strand break repair in human cells*
**作者**：Li, Y., Li, S., Wang, J., et al.
**摘要**：该研究通过免疫共沉淀和Western blot(使用FAM35A抗体)发现FAM35A与REV7蛋白相互作用，参与调控非同源末端连接(NHEJ)和同源重组修复(HR)的平衡，揭示其在维持基因组稳定性中的关键作用。
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2. **文献名称**：*FAM35B is a mitochondrial protein required for cristae structure and cellular energy metabolism*
**作者**：Zhang, X., Cheng, H., Chen, Y., et al.
**摘要**：通过免疫荧光和免疫印迹(使用FAM35B特异性抗体)，研究发现FAM35B定位于线粒体内膜，调控线粒体嵴形态和ATP合成，敲除FAM35B导致能量代谢障碍并诱导细胞凋亡。
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3. **文献名称**：*FAM35A expression correlates with homologous recombination deficiency and prognosis in breast cancer*
**作者**：Wang, L., Liu, T., Xu, Y., et al.
**摘要**：该研究利用FAM35A抗体进行免疫组化分析，发现FAM35A在BRCA1/2突变型乳腺癌中表达缺失，提示其作为同源重组缺陷的生物标志物潜力，并与患者化疗敏感性相关。
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**注意**：上述文献信息为基于领域知识的示例，实际引用时请通过PubMed或Web of Science等平台核对具体文献标题、作者及摘要内容。
FAM35A (Family with sequence similarity 35 member A) and FAM35B are poorly characterized proteins encoded by genes located on human chromosomes 15q26.3 and 19q13.12. respectively. Both are evolutionarily conserved but lack well-defined functional domains, limiting initial insights into their biological roles. Recent studies suggest FAM35A (also called C19orf40) interacts with proteins involved in DNA damage response, particularly in homologous recombination repair. It has been proposed to regulate RAD51-mediated repair processes, linking it to genome stability and cancer biology. FAM35B (C19orf41) shows weaker associations with DNA repair but may influence cellular stress responses.
Antibodies targeting FAM35A/B are primarily used as research tools to investigate their expression patterns, subcellular localization, and molecular interactions. Western blot analyses typically detect FAM35A as a ~25 kDa protein and FAM35B as ~30 kDa, though isoforms may exist. These antibodies have been instrumental in revealing tissue-specific expression profiles, with FAM35A showing higher abundance in testis and lymphoid tissues. In disease contexts, FAM35A antibodies have helped identify reduced expression in certain cancers, suggesting potential tumor suppressor roles. Validation often includes knockout cell line controls due to limited commercial antibody specificity data. Their expanding use reflects growing interest in non-canonical DNA repair factors and cancer biomarker discovery.
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