| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Khdrbs1; p62; p68; Sam68;;KHDRBS1 |
| WB Predicted band size | Calculated MW: 48 kDa ; Observed MW: 68 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human KHDRBS1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于SAM68抗体的3篇参考文献及其摘要概括：
1. **文献名称**: "The nuclear protein Sam68 is a substrate for the mitogen-activated protein kinase ERK in vivo"
**作者**: Fumagalli S. et al. (1998)
**摘要**: 研究证实SAM68是ERK激酶的底物，通过磷酸化调控其在细胞核内的功能，抗体被用于检测SAM68在信号通路中的动态定位及修饰。
2. **文献名称**: "Developmental expression of Sam68 during spermatogenesis"
**作者**: Taylor S.J. et al. (2002)
**摘要**: 利用SAM68抗体在小鼠睾丸组织中发现其在精子发生过程中高表达，提示SAM68可能参与生殖细胞RNA代谢及减数分裂调控。
3. **文献名称**: "Sam68 modulates the promoter-specific transcriptional activity of the androgen receptor"
**作者**: Paronetto M.P. et al. (2007)
**摘要**: 通过抗体免疫共沉淀发现SAM68与雄激素受体相互作用，调控前列腺癌中特定靶基因的转录活性，揭示其在激素依赖性肿瘤中的作用机制。
4. **文献名称**: "Role of the RNA-binding protein Sam68 in post-transcriptional gene regulation"
**作者**: López de Silanes I. et al. (2005)
**摘要**: 使用SAM68抗体验证其在RNA剪接及运输中的功能，证明其通过结合mRNA调控靶基因表达，影响细胞增殖和应激反应。
SAM68 (Src-associated in mitosis 68 kDa) is a RNA-binding protein belonging to the STAR (signal transduction and activation of RNA) family, initially identified through its interaction with Src tyrosine kinase during mitosis. It plays a multifaceted role in RNA metabolism, including splicing, transport, translation, and stability regulation by binding to specific RNA motifs. SAM68 contains a conserved KH (hnRNP K homology) domain essential for RNA interaction and multiple phosphorylation sites that modulate its activity in response to cellular signals, such as growth factors or stress.
This protein is implicated in diverse cellular processes, such as cell cycle progression, apoptosis, and differentiation. Studies highlight its involvement in cancer progression, particularly hormone-dependent cancers (e.g., breast and prostate), where it regulates alternative splicing of oncogenic transcripts. SAM68 also interacts with viral RNAs (e.g., HIV) and participates in viral replication.
Antibodies against SAM68 are widely used to investigate its expression, localization, and molecular interactions. They aid in detecting SAM68 in tissues or cell lines via techniques like Western blot, immunohistochemistry, or immunofluorescence. Research using these antibodies has revealed post-translational modifications (e.g., phosphorylation, methylation) that dynamically regulate SAM68’s functions. Dysregulation of SAM68 is linked to pathological conditions, making it a potential therapeutic target. These antibodies remain critical tools for exploring SAM68’s roles in RNA biology and disease mechanisms.
×
