| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CARD11; CARMA1; Carma 1; BIMP3;;CARD11 |
| WB Predicted band size | 133 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human CARD11 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于CARD11抗体的3篇参考文献，涵盖其功能、突变分析及实验应用：
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1. **文献名称**: *Oncogenic CARD11 mutations in human diffuse large B cell lymphoma*
**作者**: Lenz, G. et al.
**摘要**: 研究揭示了CARD11基因在弥漫大B细胞淋巴瘤(DLBCL)中的致癌突变，通过Western blot和免疫沉淀实验使用CARD11抗体，证实突变体导致组成性NF-κB激活，促进肿瘤发生。
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2. **文献名称**: *Germline CARD11 mutation in a patient with severe congenital B cell lymphocytosis*
**作者**: Brohl, A.S. et al.
**摘要**: 报道一例儿童B细胞白血病的CARD11胚系突变，利用CARD11抗体进行蛋白表达分析，发现突变破坏蛋白质结构，引发异常淋巴细胞增殖及信号通路失调。
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3. **文献名称**: *The role of CARD11 in T cell receptor-mediated NF-κB activation*
**作者**: Hara, H. et al.
**摘要**: 早期研究通过CARD11抗体敲除实验，证明其在T细胞受体信号中的核心作用，揭示CARD11-MALT1-BCL10复合物调控NF-κB活化的分子机制。
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**备注**：上述文献中使用的CARD11抗体主要用于检测蛋白表达(如Western blot)、定位(免疫荧光)及功能研究(如信号通路分析)，为理解CARD11在免疫疾病及癌症中的作用提供了关键实验工具。建议结合具体研究目的(如突变检测、诊断或机制探索)选择参考文献。
CARD11 (Caspase Recruitment Domain Family Member 11) is a scaffolding protein critical for antigen receptor-mediated signaling in lymphocytes. It belongs to the membrane-associated guanylate kinase (MAGUK) family and plays a key role in activating the NF-κB pathway, which regulates immune response genes. Upon T- or B-cell receptor engagement, CARD11 forms a multiprotein complex with BCL10 and MALT1 (the CBM complex), facilitating downstream signaling that drives lymphocyte activation, proliferation, and differentiation.
Mutations in the CARD11 gene are linked to immune dysregulation. Gain-of-function mutations cause constitutive NF-κB activation, associated with B-cell lymphomas and autoimmune disorders. Conversely, loss-of-function mutations result in immunodeficiency syndromes like severe combined immunodeficiency (SCID) or B-cell expansion with NF-κB and T-cell anergy (BENTA).
CARD11 antibodies are essential tools in research and diagnostics. They help detect protein expression, assess mutational impacts, and study signaling aberrations in diseases. For example, anti-CARD11 antibodies are used in Western blotting, immunohistochemistry, or flow cytometry to evaluate its expression levels or localization in lymphoid tissues. In clinical contexts, these antibodies aid in identifying pathogenic variants or monitoring therapeutic responses in lymphoma patients. Additionally, they enable mechanistic studies of CARD11’s interactions within the CBM complex, informing targeted drug development for immune disorders or cancers.
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