| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cul2; CUL 2; CUL2; cullin 2;;Cullin 2 |
| WB Predicted band size | 87 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Cullin 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Cullin2抗体的参考文献(注：文献为虚拟示例，实际引用需核实)：
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1. **文献名称**：*Cullin2-based ubiquitin ligase complex regulates hypoxia-inducible factor-1α in renal carcinoma*
**作者**：Smith J, et al.
**摘要**：本研究利用Cullin2抗体进行免疫共沉淀实验，证实Cullin2与VHL蛋白结合形成E3泛素连接酶复合体，调控HIF-1α的泛素化降解，并揭示其在肾透明细胞癌中的异常表达。
2. **文献名称**：*Antibody validation for Cullin2 in cellular localization and protein interaction studies*
**作者**：Lee H, et al.
**摘要**：通过Western blot和免疫荧光技术验证Cullin2抗体的特异性，证明其适用于检测细胞核/质分布，并用于筛选Cullin2与Elongin B/C的相互作用机制。
3. **文献名称**：*Targeting Cullin2 suppresses tumor angiogenesis in hepatocellular carcinoma*
**作者**：Zhang R, et al.
**摘要**：使用Cullin2抗体敲低实验，发现抑制Cullin2可通过阻断VEGF信号通路减少肝癌血管生成，提示其作为潜在治疗靶点。
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如需实际文献，建议通过PubMed或Google Scholar搜索关键词“Cullin2 antibody application”或“Cullin2 complex function”获取近期研究。
Cullin2 antibody is a crucial tool in studying the Cullin2 protein, a scaffold component of the Cullin-RING E3 ubiquitin ligase (CRL) complexes. Cullin2. part of the Cullin protein family, forms the CRL2 complex by binding to Rbx1 (RING box protein 1) and Elongin B/C adaptors. This complex facilitates substrate recognition and polyubiquitination, targeting proteins for proteasomal degradation. Cullin2 is notably involved in regulating hypoxia-inducible factor (HIF)-α by interacting with the Von Hippel-Lindau (VHL) tumor suppressor protein, a key mechanism in oxygen-sensing pathways. Dysregulation of Cullin2 is linked to cancer, developmental disorders, and VHL disease.
Cullin2 antibodies are widely used in Western blotting, immunoprecipitation, and immunohistochemistry to detect protein expression, localization, and interactions in cellular and tissue samples. They aid in investigating Cullin2's role in cell cycle control, DNA damage response, and tumorigenesis. Researchers also utilize these antibodies to explore therapeutic strategies targeting CRL2 complexes, particularly in cancers with VHL mutations or HIF pathway activation. Commercial Cullin2 antibodies are typically raised against specific epitopes (e.g., N-terminal or C-terminal regions) and validated for cross-reactivity across species like human, mouse, and rat. Proper controls are essential due to potential cross-reactivity with other Cullin family members.
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