| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cul4a; Cullin-4A;;Cullin 4A |
| WB Predicted band size | 88 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Cullin 4A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4条关于Cullin4A抗体的参考文献及其摘要内容概要：
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1. **文献名称**：*Cullin4A associates with the ubiquitin-like protein NEDD8 and regulates the COP9 signalosome*
**作者**：Higa LA et al.
**摘要**：该研究阐明了Cullin4A与NEDD8蛋白的结合机制，并通过特异性抗体验证其在COP9信号体(CSN)复合体中的作用，揭示了Cullin4A在泛素化途径中的调控功能。
2. **文献名称**：*CRL4Cdt2: The Architecture of a Ubiquitin Ligase*
**作者**：Jackson S, Xirodimas D.
**摘要**：作者利用Cullin4A抗体进行免疫共沉淀实验，解析了CRL4Cdt2泛素连接酶复合体的结构，并证明其在DNA损伤修复和细胞周期调控中的关键作用。
3. **文献名称**：*Cullin4A enhances neuroendocrine differentiation of prostate cancer cells*
**作者**：Zheng L et al.
**摘要**：通过免疫组化(IHC)和Western blot技术，研究发现Cullin4A在前列腺癌神经内分泌分化中高表达，其抗体被用于评估临床样本中的蛋白表达水平与预后的相关性。
4. **文献名称**：*Cullin4A interacts with HIV-1 Vpr and regulates G2/M cell cycle arrest*
**作者**：Zhuang M et al.
**摘要**：该文献使用Cullin4A抗体验证其与HIV-1病毒蛋白Vpr的相互作用，证明Cullin4A通过调控泛素化过程介导病毒感染引起的G2/M期细胞周期停滞。
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这些文献涵盖了Cullin4A抗体在机制研究、疾病模型及病毒学中的应用，可作为相关研究的参考。
The Cullin4A antibody is a crucial tool in studying the Cullin4A protein, a core component of the Cullin-RING E3 ubiquitin ligase (CRL4) complex. Cullin4A, encoded by the *CUL4A* gene, functions as a scaffold to assemble substrate receptors (e.g., DDB1) and RING-box proteins (e.g., ROC1), facilitating targeted ubiquitination of proteins for proteasomal degradation. This process regulates critical cellular pathways, including DNA repair, cell cycle progression, chromatin remodeling, and response to DNA damage. Dysregulation of Cullin4A is linked to genomic instability and diseases such as cancer, making it a focus in oncogenesis and therapeutic research.
Cullin4A antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and interaction partners. They help validate Cullin4A's role in CRL4 complexes and its involvement in disease mechanisms. Antibodies are available as monoclonal or polyclonal forms, often validated for specificity across human, mouse, and rat samples. Researchers also utilize these antibodies to explore post-translational modifications (e.g., neddylation) that regulate Cullin4A activity. Given its association with disorders like Xeroderma pigmentosum and cancer subtypes, Cullin4A antibodies are vital for both basic research and potential biomarker development.
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