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Human Lymphocyte activation gene 3 protein(LAG3) ELISA kit

  • 中文名: 人淋巴细胞活化基因3蛋白(LAG3)酶联免疫试剂盒
货号: IPD-EL012719HU
Price: ¥2500
数量:
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  • 产品描述:人淋巴细胞活化基因3蛋白(LAG3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的LAG3含量。LAG3(淋巴细胞活化基因3)是一种免疫球蛋白超家族的跨膜蛋白,主要在活化的T细胞、NK细胞等免疫细胞上表达。它通过抑制T细胞活化和增殖,参与免疫调节。研究显示,LAG3在多种肿瘤中表达,与肿瘤免疫逃逸相关,成为肿瘤免疫治疗的新靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL,适用于肿瘤微环境研究、免疫治疗药物开发中LAG3表达动态监测、自身免疫疾病模型构建等科研场景本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。

  • 别名:CD223 ELISA Kit; CD223 antigen ELISA Kit; FDC protein ELISA Kit; LAG-3 ELISA Kit; Lag3 ELISA Kit; LAG3_HUMAN ELISA Kit; Lymphocyte activating 3 ELISA Kit; Lymphocyte activation gene 3 ELISA Kit; Lymphocyte activation gene 3 protein ELISA Kit; Protein FDC ELISA Kit

  • 缩写:LAG3

  • Uniprot No.:P18627

  • 种属:Homo sapiens (Human)

  • 样本类型:serum, plasma, tissue homogenates

  • 检测范围:0.156 ng/mL-10 ng/mL

  • 灵敏度:0.039 ng/mL

  • 反应时间:1-5h

  • 样本体积:50-100ul

  • 检测波长:450 nm

  • 研究领域:Immunology

  • 测定原理:quantitative

  • 测定方法:Sandwich

  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human LAG3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    SampleSerum(n=4)
    1:20Average %98
    Range %92-105
    1:40Average %95
    Range %87-100
    1:80Average %98
    Range %90-105
    1:160Average %94
    Range %89-99
  • 回收率:

    The recovery of human LAG3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9489-98
    EDTA plasma (n=4)9692-105














  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected102.9582.8792.9192.70152.4692.3742.4222.2042.51.6841.7261.7051.4871.251.0331.0671.0500.8320.6250.6750.6620.6690.4510.3120.4650.4600.4630.2450.1560.3220.3450.3340.11600.2170.2190.218 






  • 货期:3-5 working days

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