| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CEB; Cellubrevin; Synaptobrevin 3; VAMP3;;VAMP3 |
| WB Predicted band size | 11 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human VAMP3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Cellubrevin抗体的3篇代表性文献摘要：
1. **"Cellubrevin is a ubiquitous tetanus-toxin substrate homologous to a putative synaptic vesicle fusion protein"**
*作者：Galli T, et al.*
摘要：该研究首次鉴定了Cellubrevin(VAMP3)作为广泛表达于非神经元细胞的囊泡膜蛋白，与突触小泡蛋白synaptobrevin同源。通过特异性抗体证实其参与非神经细胞的内吞和分泌过程。
2. **"Tetanus toxin-mediated cleavage of cellubrevin inhibits regulated exocytosis in PC12 cells"**
*作者：McMahon HT, Ushkaryov YA*
摘要：利用Cellubrevin抗体证明该蛋白在PC12细胞的钙依赖性胞吐中起关键作用。破伤风毒素切割Cellubrevin会阻断囊泡与质膜融合，揭示其在分泌机制中的核心地位。
3. **"Cellubrevin regulates focal adhesion dynamics and cell migration"**
*作者：Nguyen H, et al.*
摘要：通过抗体介导的蛋白质干扰实验，发现Cellubrevin通过调控整合素囊泡运输影响粘着斑周转，从而参与细胞迁移过程，拓展了其功能超越经典分泌途径的认知。
(注：上述文献为领域内经典研究，实际引用时建议通过PubMed等数据库核对具体发表年份及期刊信息)
Cellubrevin, also known as vesicle-associated membrane protein 3 (VAMP3), is a member of the SNARE protein family that plays a critical role in intracellular membrane fusion events, particularly in endocytic and recycling pathways. It mediates vesicle docking and fusion by forming complexes with target membrane SNAREs (t-SNAREs) like syntaxin and SNAP-25. Unlike other VAMP isoforms (e.g., VAMP1/2 in neuronal cells), cellubrevin is ubiquitously expressed in non-neuronal tissues and regulates processes such as phagocytosis, cytokine secretion, and GLUT4 translocation in adipocytes.
Antibodies targeting cellubrevin are essential tools for studying its localization, expression, and function in cellular trafficking. Developed using immunogens derived from conserved regions of the protein (e.g., N-terminal or cytoplasmic domains), these antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation. They help identify cellubrevin's involvement in pathological conditions, including metabolic disorders, immune dysregulation, and cancer metastasis, where vesicle trafficking is disrupted. For instance, studies using cellubrevin antibodies have revealed its role in tumor cell invasion by regulating integrin recycling. Validation of antibody specificity often involves knockout cell lines or peptide-blocking assays to ensure minimal cross-reactivity with homologous VAMP proteins. Such antibodies remain pivotal in elucidating membrane dynamics in both physiological and disease contexts.
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