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产品描述：人神经细胞粘附分子配体1(NCAM-L1/CD171)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的L1CAM含量。L1CAM是一种免疫球蛋白超家族成员，主要参与神经细胞生长和功能调控。其跨膜胞外域可被切割释放可溶性L1CAM-ECD，影响自身或周围环境。研究显示，L1CAM在肿瘤中发挥重要作用，如增强肿瘤细胞粘附、迁移和侵袭能力，并可能通过调节下游信号通路促进肿瘤进展。试剂盒检测范围为1.25 ng/mL-80 ng/mL，作为科研工具，其适用于神经生物学、肿瘤微环境、神经退行性疾病机制等领域的研究，例如通过检测不同病理模型中NCAM-L1的表达动态，探究其与神经再生、肿瘤侵袭转移或免疫调控的分子关联，也可用于体外培养细胞或动物模型样本的定量分析，为神经发育、细胞信号传导等基础研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Antigen identified by monoclonal ELISA Kit R1 ELISA Kit; CAML1 ELISA Kit; CD171 ELISA Kit; CD171 antigen ELISA Kit; HSAS ELISA Kit; HSAS1 ELISA Kit; Hyd ELISA Kit; L1 ELISA Kit; L1 cell adhesion molecule ELISA Kit; L1-NCAM ELISA Kit; L1cam ELISA Kit; L1CAM_HUMAN ELISA Kit; MASA ELISA Kit; MIC5 ELISA Kit; N CAML1 ELISA Kit; N-CAM-L1 ELISA Kit; NCAM-L1 ELISA Kit; NCAML1 ELISA Kit; Nerve-growth factor-inducible large external glycoprotein ELISA Kit; Neural cell adhesion molecule L1 ELISA Kit; NILE ELISA Kit; OTTHUMP00000025992 ELISA Kit; S10 ELISA Kit; SPG1 ELISA Kit
缩写：L1CAM
Uniprot No.：P32004
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NCAM-L1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 86-92 | |||||
| 1:2 | Average % | 107 | ||||
| Range % | 99-111 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of human NCAM-L1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 84-95 | ||||
| EDTA plasma (n=4) | 104 | 95-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.0141.9942.0041.902401.4291.4051.4171.315200.8630.8440.8540.752100.4770.4650.4710.36950.2810.2870.2840.1822.50.2250.2330.2290.1271.250.1560.1520.1540.05200.1030.1010.102 | ||||||
货期：3-5 working days
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