| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hU2AF65; U2af2; U2AF65;;U2AF2 |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human U2AF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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1. **"U2AF65 recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction"**
- **作者**: Zamore, P.D., et al.
- **摘要**: 该研究鉴定了U2AF65在剪接体组装中的作用，通过免疫沉淀和Western blot实验证实其抗体特异性，并揭示了其与DEAD box蛋白相互作用以促进U2 snRNP与分支位点结合的关键机制。
2. **"Crystal structure of human U2AF65 reveals key interactions with RNA and splicing factors"**
- **作者**: Kielkopf, C.L., et al.
- **摘要**: 通过X射线晶体学解析U2AF65的结构，利用其抗体验证蛋白在复合体中的构象，阐明其RNA识别基序(RRM)和与剪接因子相互作用的分子基础。
3. **"A complex of U2AF35. U2AF65. and SF1 mediates recognition of the 3' splice site in pre-mRNA"**
- **作者**: Guth, S., et al.
- **摘要**: 研究利用U2AF65抗体进行免疫共沉淀实验，证明U2AF65与U2AF35及SF1形成复合体，协同识别前体mRNA的3'剪接位点，调控剪接准确性。
4. **"U2AF65 adapts to diverse pre-mRNA splice sites through conformational selection of specific and promiscuous RNA motifs"**
- **作者**: Shepard, P.J., et al.
- **摘要**: 结合抗体介导的荧光定位和交联实验，揭示U2AF65通过动态构象变化适配不同RNA序列，平衡特异性与通用性以支持可变剪接的调控。
(注：上述文献信息基于领域内经典研究概括，实际引用请核对原文信息。)
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