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产品描述：人赖氨酸特异性脱甲基酶5A(JARID1A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的KDM5A含量。KDM5A 是一个重要靶点。它是组蛋白去甲基化酶，参与基因表达调控等重要生物学过程。研究显示其在肿瘤发生、发展中起关键作用，干扰其活性或表达有望成为肿瘤治疗新思路，目前围绕它的作用机制及靶向药物研发是研究热点。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于肿瘤机制研究、表观遗传学调控探索、药物靶点筛选等科研领域，为探究JARID1A在疾病模型中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Histone demethylase JARID1A ELISA Kit; JARID1A ELISA Kit; Jumonji/ARID domain containing protein 1A ELISA Kit; Jumonji/ARID domain-containing protein 1A ELISA Kit; Kdm5a ELISA Kit; KDM5A_HUMAN ELISA Kit; Lysine-specific demethylase 5A ELISA Kit; RBBP-2 ELISA Kit; RBBP2 ELISA Kit; RBP2 ELISA Kit; Retinoblastoma binding protein 2 ELISA Kit; Retinoblastoma-binding protein 2 ELISA Kit
缩写：KDM5A
Uniprot No.：P29375
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KDM5A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 83-92 | |||||
回收率：
| The recovery of human KDM5A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 15002.3672.2722.3202.229 7502.0522.0062.0291.938 3751.5931.5341.5641.473 187.51.1941.1091.1521.061 940.6890.6740.6820.591 470.3190.3410.3300.239 23.50.1690.1750.1720.081 00.0870.0940.091 | ||||||
货期：3-5 working days
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