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产品描述：人Jagged-1蛋白-1(JAG1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的JAG1含量。JAG1是一种重要的靶点。它是Notch信号通路的配体，在胚胎发育、细胞增殖与分化等过程中起关键作用。研究发现其异常激活与肿瘤发生发展相关，目前围绕JAG1开展了针对肿瘤治疗的相关机制研究，旨在阻断其信号传导来抑制肿瘤。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本产品适用于基础科研领域，如探究疾病模型中JAG1的表达调控机制、评估药物干预对Notch信号通路的影响，或用于细胞因子网络互作研究，为心血管发育、肿瘤微环境调控等方向的体外实验提供可靠分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AGS ELISA Kit; AHD ELISA Kit; AWS ELISA Kit; CD 339 ELISA Kit; CD339 ELISA Kit; CD339 antigen ELISA Kit; Headturner ELISA Kit; hJ1 ELISA Kit; Htu ELISA Kit; Jag 1 ELISA Kit; Jag1 ELISA Kit; JAG1_HUMAN ELISA Kit; Jagged 1 ELISA Kit; Jagged1 (Alagille syndrome) ELISA Kit; Jagged1 ELISA Kit; JAGL1 ELISA Kit; MGC104644 ELISA Kit; OTTHUMP00000030278 ELISA Kit; Protein jagged-1 ELISA Kit; Ser 1 ELISA Kit; Ser1 ELISA Kit; Serrate 1 ELISA Kit; Slalom ELISA Kit
缩写：JAG1
Uniprot No.：P78504
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human JAG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 100 | |||||
| Range % | 93-105 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 90-101 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 88-102 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 88-102 | ||||||
回收率：
| The recovery of human JAG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 83-96 | |||||
| EDTA plasma (n=4) | 99 | 89-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected101.9931.9121.9531.77351.4851.5091.4971.3172.50.9751.0080.9920.8121.250.6610.6450.6530.4730.6250.4010.3960.3990.2190.3120.3050.3170.3110.1310.1560.2410.2500.2460.06600.1820.1780.180 | |||||||
货期：3-5 working days
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