| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Capsulin; Epicardin; POD 1; POD1; TCF-21; tcf21;;TCF21 |
| WB Predicted band size | Calculated MW: 20 kDa ; Observed MW: 26 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human TCF21 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于TCF21抗体的3篇参考文献示例(注：以下内容为虚构示例，仅供参考格式)：
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1. **文献名称**：*TCF21 Expression in Cardiac Progenitor Cells Regulates Heart Development*
**作者**：Lu, J. et al.
**摘要**：本研究利用TCF21抗体进行免疫组化分析，揭示了TCF21在胚胎心脏祖细胞中的特异性表达模式。实验表明，TCF21缺失会导致小鼠心脏间隔发育异常，提示其在心脏形态发生中的关键作用。
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2. **文献名称**：*TCF21 as a Tumor Suppressor in Non-Small Cell Lung Cancer*
**作者**：Smith, R. et al.
**摘要**：通过TCF21抗体的免疫组织化学检测，发现TCF21在肺癌组织中表达显著下调。功能实验证实TCF21通过调控上皮-间质转化(EMT)抑制肿瘤转移，提示其作为肺癌预后标志物的潜力。
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3. **文献名称**：*Antibody Validation for TCF21 in Human Renal Tissues*
**作者**：Garcia, M. et al.
**摘要**：本研究系统评估了TCF21抗体在人肾脏组织中的特异性，通过Western blot和免疫荧光验证其与目标蛋白的结合能力。结果显示该抗体在肾小球足细胞中具有高特异性，适用于病理学研究。
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(注：如需真实文献，请通过PubMed或Google Scholar检索关键词“TCF21 antibody”获取。)
The TCF21 antibody is a crucial tool in studying the role of Transcription Factor 21 (TCF21), a basic helix-loop-helix (bHLH) transcription factor implicated in organ development and disease. TCF21. also known as Pod1 or Capsulin, regulates cell differentiation and lineage commitment, particularly in mesenchymal cells during embryogenesis. It is essential for the development of organs like the heart, kidneys, and lungs, where it influences cell fate decisions and tissue patterning. In adulthood, TCF21 is linked to pathological processes, including cancer, fibrosis, and atherosclerosis, where its expression often correlates with tumor suppression or disease progression.
Researchers use TCF21 antibodies primarily in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein expression, localization, and dynamics in tissues or cell lines. These antibodies are vital for investigating TCF21's regulatory mechanisms, such as its interaction with epigenetic modifiers or its role in epithelial-mesenchymal transition (EMT). Commercially available TCF21 antibodies are typically raised in rabbits or mice, validated for specificity via knockout controls, and optimized for various sample types. Recent studies highlight TCF21's potential as a biomarker in cancers (e.g., lung, breast) or cardiovascular diseases, driving interest in therapeutic targeting. However, variability in antibody performance across experimental conditions necessitates careful validation to ensure reproducibility in research.
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