首页 / 产品 / 抗体 / 一抗

Rabbit Monoclonal DGAT1 Antibody

  • 中文名: DGAT1抗体
  • 别    名: ARAT; ARGP1; C75990; Dgat; DGAT1; DIAR7; Diglyceride acyltransferase;;DGAT1
货号: IPDX18128
Price: ¥1280
数量:
大包装询价

验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20-1/50 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesARAT; ARGP1; C75990; Dgat; DGAT1; DIAR7; Diglyceride acyltransferase;;DGAT1
WB Predicted band sizeCalculated MW: 55 kDa ; Observed MW: 48 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthesized peptide derived from human DGAT1
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

+ +

参考文献

以下是关于DGAT1抗体的3篇参考文献示例,包括文献名称、作者及摘要内容概括:

---

1. **文献名称**: "DGAT1 Antibody-Based Detection of Enzyme Expression in Adipose Tissue: Implications for Obesity Research"

**作者**: Smith A, et al.

**摘要**: 本研究利用特异性DGAT1抗体,通过Western blot和免疫组化技术,揭示了DGAT1在人类和小鼠脂肪组织中的高表达水平。结果表明,DGAT1表达量与肥胖程度呈正相关,提示其作为代谢疾病治疗靶点的潜力。

---

2. **文献名称**: "Development of a Monoclonal Antibody Targeting DGAT1 for Functional Studies in Lipid Metabolism"

**作者**: Chen L, et al.

**摘要**: 作者开发了一种高特异性抗DGAT1单克隆抗体,用于研究DGAT1在肝细胞脂质代谢中的功能。通过免疫沉淀和细胞定位实验,发现DGAT1活性受饮食调控,抑制其功能可减少脂滴形成,为治疗非酒精性脂肪肝提供依据。

---

3. **文献名称**: "DGAT1 Knockout Validation Using CRISPR/Cas9 and Antibody-Based Protein Detection"

**作者**: Brown K, et al.

**摘要**: 该研究通过CRISPR/Cas9技术构建DGAT1基因敲除小鼠模型,并利用商业化DGAT1抗体验证蛋白缺失。结果表明,敲除小鼠表现出显著降低的甘油三酯水平,证实DGAT1在脂质合成中的关键作用。

---

注:以上文献为示例性内容,实际引用时需根据真实发表的论文进行核实。如需查找真实文献,可检索PubMed、Google Scholar等平台,关键词为“DGAT1 antibody”“DGAT1 immunodetection”等。

背景信息

The DGAT1 (diacylglycerol O-acyltransferase 1) antibody is a key tool for studying the enzyme DGAT1. which catalyzes the final step of triglyceride synthesis by transferring acyl groups to diacylglycerol. DGAT1 is a membrane-bound protein predominantly located in the endoplasmic reticulum and plays a critical role in lipid metabolism, energy storage, and cellular lipid droplet formation. It is expressed in tissues with high lipid activity, such as adipose tissue, liver, and intestine. Research on DGAT1 has implications for understanding metabolic disorders, including obesity, diabetes, and non-alcoholic fatty liver disease (NAFLD), as well as its role in intestinal lipid absorption and secretion. DGAT1 knockout studies in mice reveal resistance to diet-induced obesity but also highlight its essential function in neonatal development and lactation. Antibodies targeting DGAT1 are widely used in Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression, localization, and regulation under various physiological or pathological conditions. They also aid in evaluating therapeutic strategies, such as DGAT1 inhibitors, for metabolic diseases. These antibodies are typically validated for specificity across species (e.g., human, mouse, rat) and may target specific epitopes or isoforms. Understanding DGAT1's function through antibody-based assays contributes to insights into lipid homeostasis and related therapeutic developments.

客户数据及评论

折叠内容

大包装询价

×