WB | 咨询技术 | Human,Mouse,Rat |
IF | 1/20-1/50 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 1/50-1/200 | Human,Mouse,Rat |
FCM | 1/20-1/100 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | ARAT; ARGP1; C75990; Dgat; DGAT1; DIAR7; Diglyceride acyltransferase;;DGAT1 |
WB Predicted band size | Calculated MW: 55 kDa ; Observed MW: 48 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | A synthesized peptide derived from human DGAT1 |
Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于DGAT1抗体的3篇参考文献示例,包括文献名称、作者及摘要内容概括:
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1. **文献名称**: "DGAT1 Antibody-Based Detection of Enzyme Expression in Adipose Tissue: Implications for Obesity Research"
**作者**: Smith A, et al.
**摘要**: 本研究利用特异性DGAT1抗体,通过Western blot和免疫组化技术,揭示了DGAT1在人类和小鼠脂肪组织中的高表达水平。结果表明,DGAT1表达量与肥胖程度呈正相关,提示其作为代谢疾病治疗靶点的潜力。
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2. **文献名称**: "Development of a Monoclonal Antibody Targeting DGAT1 for Functional Studies in Lipid Metabolism"
**作者**: Chen L, et al.
**摘要**: 作者开发了一种高特异性抗DGAT1单克隆抗体,用于研究DGAT1在肝细胞脂质代谢中的功能。通过免疫沉淀和细胞定位实验,发现DGAT1活性受饮食调控,抑制其功能可减少脂滴形成,为治疗非酒精性脂肪肝提供依据。
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3. **文献名称**: "DGAT1 Knockout Validation Using CRISPR/Cas9 and Antibody-Based Protein Detection"
**作者**: Brown K, et al.
**摘要**: 该研究通过CRISPR/Cas9技术构建DGAT1基因敲除小鼠模型,并利用商业化DGAT1抗体验证蛋白缺失。结果表明,敲除小鼠表现出显著降低的甘油三酯水平,证实DGAT1在脂质合成中的关键作用。
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注:以上文献为示例性内容,实际引用时需根据真实发表的论文进行核实。如需查找真实文献,可检索PubMed、Google Scholar等平台,关键词为“DGAT1 antibody”“DGAT1 immunodetection”等。
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