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产品描述：人Anosmin-1(KAL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的KAL1含量。KAL1（Kallmann综合征1）是一种罕见的遗传性疾病，主要与嗅觉缺失或减退和性腺功能减退有关。其研究机制主要涉及KAL1基因突变导致嗅觉和GnRH神经元迁移异常，进而引起泌尿生殖系统异常和联带运动。KAL1基因突变与X连锁KS表型相关，其表型似乎比其他分子缺陷更为严重。试剂盒检测范围为125 pg/mL-8000 pg/mL，本试剂盒适用于科研领域中对Anosmin-1表达水平的分析，例如探究Kallmann综合征病理机制、生殖内分泌学研究或神经发育相关模型构建。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Adhesion molecule-like X-linked ELISA Kit; ADMLX ELISA Kit; Anosmin-1 ELISA Kit; HHA ELISA Kit; KAL ELISA Kit; KAL1 ELISA Kit; KALIG 1 ELISA Kit; KALIG1 ELISA Kit; Kallmann syndrome 1 sequence (anosmin 1) ELISA Kit; Kallmann syndrome 1 sequence ELISA Kit; Kallmann syndrome interval gene 1 ELISA Kit; Kallmann syndrome protein ELISA Kit; KALM ELISA Kit; KALM_HUMAN ELISA Kit; KMS ELISA Kit; OTTHUMP00000022873 ELISA Kit
缩写：KAL1
Uniprot No.：P23352
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KAL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 92-104 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of human KAL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 94-104 | ||||
| EDTA plasma (n=4) | 96 | 91-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.4872.4662.4772.37540002.1912.1802.1862.08420001.8281.8561.8421.74010001.3551.3971.3761.2745000.9620.9910.9770.8752500.5540.5940.5740.4721250.2980.2810.2900.18800.1010.1030.102 | ||||||
货期：3-5 working days
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