| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HB58; Hbeta58; hVPS26; PEP8A; VPS 26; VPS26A;;VPS26A |
| WB Predicted band size | 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human VPS26A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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1. **"Retromer: A Master Conductor of Endocytic Sorting"**
- Authors: Seaman, M.N.J.
- 摘要：本文综述了Retromer复合体(含VPS26、VPS29和VPS35)在内体分选和膜运输中的核心作用，重点讨论了VPS26作为关键亚基如何识别货物蛋白并调控逆向运输途径。研究还提及VPS26A和VPS26B亚型的功能差异及其抗体的应用验证。
2. **"Structural basis for recruitment of the endosomal retromer complex by the WASH complex"**
- Authors: Harbour, M.E., et al.
- 摘要：通过冷冻电镜解析VPS26A与WASH复合体相互作用的结构，揭示了VPS26A在连接Retromer与细胞骨架调控蛋白中的分子机制。研究使用特异性抗体验证VPS26A在细胞内的定位及功能缺失表型。
3. **"VPS26B promotes melanoma progression via retromer-independent metabolic reprogramming"**
- Authors: Zhang, Y., et al.
- 摘要：研究发现VPS26B通过非依赖Retromer的途径调控黑色素瘤细胞的糖酵解和增殖，并通过免疫印迹和免疫组化实验验证其抗体特异性，提示VPS26B可能成为癌症治疗新靶点。
4. **"Altered retromer-mediated sorting in Alzheimer's disease"**
- Authors: Small, S.A., et al.
- 摘要：探讨VPS26A在阿尔茨海默病中的表达异常及其与淀粉样蛋白前体(APP)运输的关联，利用VPS26A抗体证明其在患者脑组织中的表达下降，提示Retromer功能障碍可能参与神经退行性病变。
VPS26 antibodies are essential tools for studying the VPS26 protein family, which comprises two paralogs, VPS26A and VPS26B. These proteins are key components of the retromer complex, a conserved eukaryotic machinery critical for endosomal protein sorting and trafficking. The retromer, composed of a VPS26-VPS29-VPS35 trimer and sorting nexin proteins, mediates retrograde transport of cargo from endosomes to the trans-Golgi network or plasma membrane, ensuring proper cellular homeostasis. VPS26A is the predominant isoform, widely expressed and involved in recycling receptors like cation-independent mannose 6-phosphate receptor (CI-MPR). VPS26B, though structurally similar, exhibits distinct tissue expression and may regulate specialized trafficking pathways or compensate for VPS26A loss.
Antibodies targeting VPS26 are used to investigate its expression, localization, and interactions in diverse contexts, including neurodegenerative diseases (e.g., Alzheimer’s and Parkinson’s), where retromer dysfunction links to pathogenic protein accumulation. They also aid in studying cancer, as altered retromer activity impacts growth factor signaling and metastasis. Researchers employ VPS26 antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to dissect retromer biology. Specificity validation (e.g., knockout controls) is crucial due to high homology between VPS26A/B. These reagents remain vital for understanding intracellular trafficking mechanisms and developing therapeutic strategies targeting retromer-related disorders.
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