首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人套膜蛋白(iNV)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、组织匀浆样本中的IVL含量。人套膜蛋白(iNV)是一种重要的细胞膜蛋白，其背景研究涉及其在细胞信号传导和细胞结构维持中的关键作用。研究机制主要关注其与细胞内其他蛋白的相互作用，以及通过ELISA等检测方法对其浓度进行定量分析。试剂盒检测范围为1.25 ng/mL-80 ng/mL，可满足科研场景中对病毒感染机制、抗病毒药物筛选、疫苗研发等方向的研究需求，支持体外细胞模型、动物实验样本及病理组织样本的定量检测，为病毒学基础研究及生物医学应用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：INVO_HUMAN ELISA Kit; Involucrin ELISA Kit; IVL ELISA Kit
缩写：IVL
Uniprot No.：P07476
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human iNV in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 95-109 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of human iNV spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.9152.7502.8332.666402.2102.1322.1712.004201.5611.4761.5191.352100.9510.9970.9740.80750.6160.6020.6090.4422.50.3960.4270.4120.2451.250.2740.2580.2660.09900.1620.1710.167 | ||||||
货期：3-5 working days
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