| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hVPS18; PEP3; vps18;;VPS18 |
| WB Predicted band size | Calculated MW: 110 kDa ; Observed MW: 100 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human VPS18 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是3篇与VPS18抗体相关的假设文献示例(请注意文献为虚构，仅供格式参考)：
1. **标题**: "VPS18 regulates lysosomal trafficking via HOPS complex assembly"
**作者**: Smith J, et al.
**摘要**: 本研究利用VPS18特异性抗体，通过免疫共沉淀和Western blot证实了VPS18在HOPS复合体形成中的关键作用，并发现其缺失导致溶酶体酶运输缺陷。
2. **标题**: "Antibody-based profiling of VPS18 in neurodegenerative models"
**作者**: Chen L, et al.
**摘要**: 开发了一种高特异性VPS18单克隆抗体，应用于阿尔茨海默病模型小鼠脑组织，揭示了VPS18表达水平与tau蛋白异常聚集的相关性。
3. **标题**: "CRISPR screening identifies VPS18 as a mediator of autophagosome-lysosome fusion"
**作者**: González A, et al.
**摘要**: 通过CRISPR筛选结合VPS18抗体标记的免疫荧光成像，证明VPS18缺失会阻断自噬体-溶酶体膜融合过程，影响细胞自噬稳态。
(注：实际文献需通过PubMed/Google Scholar检索确认，建议使用关键词“VPS18 antibody”或“VPS18 HOPS”配合“immunoblotting/immunofluorescence”等实验方法进行精准查询。)
The VPS18 antibody is a research tool designed to detect VPS18 (Vacuolar Protein Sorting 18), a critical component of cellular trafficking pathways. VPS18 is part of the HOPS (Homotypic Fusion and Vacuole Protein Sorting) complex, which mediates membrane fusion events in endolysosomal trafficking and autophagosome-lysosome fusion. It plays a vital role in lysosomal function, vesicle transport, and organelle biogenesis, impacting processes like autophagy, endocytosis, and protein degradation. Dysregulation of VPS18 is linked to lysosomal storage disorders, neurodegenerative diseases, and immune deficiencies.
The antibody is commonly used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to study VPS18 expression, localization, and interactions. It helps researchers investigate how VPS18 mutations or altered expression affect lysosomal activity, cellular homeostasis, and disease mechanisms. Many VPS18 antibodies are validated in models like human cell lines or murine tissues, with specificity confirmed via knockout controls.
As a reagent in cell biology and disease research, the VPS18 antibody contributes to understanding intracellular trafficking defects and developing therapeutic strategies for related disorders. Commercial versions are available from multiple suppliers, often with species-specific reactivity (e.g., human, mouse). Proper validation remains essential due to potential cross-reactivity with homologous proteins.
×
