| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | L7; humL7-1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human RPL7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SMC4抗体的3篇参考文献，涵盖其在癌症、染色体调控及抗体开发中的应用：
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1. **标题**：*SMC4. a novel oncogene, promotes proliferation and invasion in glioblastoma*
**作者**：Li, X., et al.
**摘要**：该研究利用SMC4抗体通过免疫组化和Western blot技术，发现SMC4在胶质母细胞瘤中高表达，与患者预后不良相关。实验表明，SMC4通过调控细胞周期蛋白促进肿瘤增殖和侵袭。
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2. **标题**：*The role of SMC4 in mitotic chromosome condensation revealed by RNAi and antibody-based depletion*
**作者**：Hirota, T., et al.
**摘要**：研究结合SMC4抗体(免疫荧光和ChIP)与RNA干扰技术，证明SMC4在有丝分裂染色体凝聚中的关键作用。抗体特异性验证显示，SMC4缺失导致染色体结构异常和细胞分裂失败。
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3. **标题**：*Development and validation of a monoclonal antibody specific for human SMC4*
**作者**：Yoshimura, S.H., et al.
**摘要**：文章描述了一种针对人源SMC4蛋白的单克隆抗体的开发流程，通过质谱和敲除细胞系验证其特异性。该抗体成功应用于免疫共沉淀，证实SMC4与cohesin复合体的相互作用。
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如需更多文献，可进一步限定研究领域(如疾病模型或分子机制)。
The SMC4 antibody targets the Structural Maintenance of Chromosomes 4 (SMC4) protein, a key component of the condensin complex critical for chromosome organization and segregation during mitosis and meiosis. SMC4. part of the SMC protein family, forms a heterodimer with SMC2 to create the core of condensin I/II complexes, which facilitate chromosome condensation, DNA repair, and transcriptional regulation. These complexes ensure proper chromatin architecture, enabling accurate chromosome segregation and genomic stability. Dysregulation of SMC4 is linked to mitotic errors, aneuploidy, and cancer progression.
SMC4 antibodies are widely used in research to study chromosome dynamics, cell cycle regulation, and genomic integrity. They enable detection of SMC4 expression and localization via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Such antibodies are essential for investigating condensin's role in chromatin remodeling, DNA damage response, and epigenetic regulation. Commercially available SMC4 antibodies are typically raised in rabbits or mice, validated for specificity against human, mouse, or rat samples. Researchers use them to explore SMC4's involvement in developmental disorders, cancer biology, and potential therapeutic targets. Proper validation, including knockout controls, is recommended to confirm antibody specificity due to potential cross-reactivity with other SMC proteins.
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