关键信息
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基因名
DCLK1
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简介
The DCLK1 protein is a possible kinase that may play a role in calcium signaling pathways that regulate neuronal migration during brain development. Its association suggests a key role in coordinating processes critical to normal brain formation. DCLK1 Protein, Human (sf9, His-GST) is the recombinant human-derived DCLK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- 应用
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别名
Serine/threonine-protein kinase DCLK1; Doublecortin-like kinase 1; DCAMKL1; DCDC3A
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种属
Human
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表达系统
Baculovirus
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标签
N-His;N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O15075-1
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表达区间
M1-V705
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蛋白长度
Partial
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分子量
105 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
DCLK1 (Doublecortin-like kinase 1) is a serine/threonine kinase that has gained significant attention in recent years due to its potential role in various cellular processes, including neuronal differentiation, proliferation, and migration. Initially identified for its implications in neurological disorders and its involvement in neuronal development, DCLK1 has also been linked to tumorigenesis in various cancers, particularly in pancreatic and colorectal cancer, where it is associated with poor prognosis. Its dual function as a marker for neural stem cells and its role in cancer stem cell dynamics highlight its importance in both neurobiology and oncology. Research has demonstrated that DCLK1 affects key signaling pathways, influencing cellular behavior and response to treatment. Furthermore, studies suggest that DCLK1 may serve as a therapeutic target or biomarker, providing insights into disease mechanisms and potential interventions. Given its multifaceted roles in both normal and pathological states, the development and characterization of DCLK1 recombinant proteins are crucial for further elucidating its function, exploring its interactions with other proteins, and assessing its potential for targeted therapies. Understanding DCLK1's mechanisms through recombinant protein studies could pave the way for novel diagnostic and therapeutic strategies in treating neurological disorders and cancers associated with DCLK1 dysregulation.
