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Rabbit Polyclonal SLC35B1 Antibody

  • 中文名: SLC35B1抗体
  • 别    名: AXER; UGTREL1
货号: IPDX13799
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesAXER; UGTREL1
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenSynthetic peptide of human SLC35B1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于SLC35B1抗体的示例参考文献(注:以下内容为模拟生成,具体文献需通过学术数据库验证):

1. **文献名称**: "SLC35B1 regulates sulfation of glycosaminoglycans through nucleotide sugar transport in the Golgi apparatus"

**作者**: Ishida N, Kawakita M

**摘要**: 本研究利用SLC35B1特异性抗体,通过免疫荧光和Western blot分析,揭示了SLC35B1作为3'-磷酸腺苷-5'-磷酸硫酸(PAPS)转运蛋白的功能,证明其在高尔基体硫酸软骨素合成中的关键作用。

2. **文献名称**: "SLC35B1 as a potential biomarker in hepatocellular carcinoma: Immunohistochemical analysis"

**作者**: Zhang Y, et al.

**摘要**: 通过免疫组化技术结合SLC35B1抗体,研究发现肝癌组织中SLC35B1表达显著上调,且与患者预后不良相关,提示其可能成为肝癌诊断和治疗的新靶点。

3. **文献名称**: "Functional characterization of SLC35B1 knockout cells using CRISPR/Cas9 and antibody-based validation"

**作者**: Wang L, et al.

**摘要**: 利用CRISPR/Cas9技术构建SLC35B1敲除细胞系,通过抗体验证蛋白缺失,发现SLC35B1缺失导致细胞外基质成分异常,影响细胞迁移和侵袭能力。

4. **文献名称**: "SLC35B1 interacts with HSP90 in colorectal cancer: Co-immunoprecipitation analysis"

**作者**: Tanaka R, et al.

**摘要**: 使用SLC35B1抗体进行免疫共沉淀实验,发现其与热休克蛋白HSP90存在物理相互作用,可能通过调控蛋白稳定性参与结直肠癌的耐药机制。

**提示**:以上文献为示例,实际引用需通过PubMed、Google Scholar等平台以"SLC35B1 antibody"为关键词检索,并核实实验方法和结论的准确性。

背景信息

The SLC35B1 antibody is a research tool designed to detect and study the solute carrier family 35 member B1 (SLC35B1), a nucleotide-sugar transporter localized to the endoplasmic reticulum. SLC35B1 facilitates the transport of 3'-phosphoadenosine 5'-phosphosulfate (PAPS), a critical sulfate donor required for sulfation reactions in glycosaminoglycan biosynthesis and post-translational protein modifications. This protein plays a vital role in cellular processes such as extracellular matrix organization, cell adhesion, and inflammatory responses. Dysregulation of SLC35B1 has been implicated in pathologies including cancer, metabolic disorders, and connective tissue diseases. The antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate SLC35B1 expression, subcellular localization, and functional interactions. As a member of the SLC35 transporter family, SLC35B1’s activity impacts sulfation-dependent pathways, making its study essential for understanding cellular sulfation biology and related therapeutic targets. Commercial SLC35B1 antibodies are typically raised in rabbits or mice, targeting specific epitopes within the protein’s conserved regions. Validation parameters include specificity testing via knockout controls and application-specific performance verification.

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