| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM73 |
| Uniprot No | Q86UV7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-250 aa |
| 活性数据 | MAWQVSLLEL EDRLQCPICL EVFKESLMLQ CGHSYCKGCL VSLSYHLDTK VRCPMCWQVV DGSSSLPNVS LAWVIEALRL PGDPEPKVCV HHRNPLSLFC EKDQELICGL CGLLGSHQHH PVTPVSTVCS RMKEELAALF SELKQEQKKV DELIAKLVKN RTRIVNESDV FSWVIRREFQ ELRHPVDEEK ARCLEGIGGH TRGLVASLDM QLEQAQGTRE RLAQAECVLE QFGNEDHHEF IWKFHSMASR |
| 分子量 | 28.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于TRIM73蛋白的研究目前较为有限，以下为基于TRIM蛋白家族共性及现有文献结构的模拟示例(实际引用请通过学术数据库验证)：
1. **"TRIM73 functions as an E3 ubiquitin ligase in lung cancer progression"**
*Authors: Zhang, Y. et al.*
摘要：研究揭示TRIM73在非小细胞肺癌中高表达，通过泛素化降解抑癌蛋白p53.促进肿瘤细胞增殖和转移。
2. **"The role of TRIM73 in spermatogenesis: Insights from knockout mouse models"**
*Authors: Chen, L. et al.*
摘要：利用基因敲除技术发现TRIM73对精子形成关键，缺失导致小鼠雄性不育，可能与调控减数分裂相关蛋白稳定性有关。
3. **"Structural analysis of TRIM73 reveals conserved motifs in the TRIM family"**
*Authors: Smith, J. & Wang, H.*
摘要：通过晶体结构解析，阐明TRIM73的RING、B-box和 coiled-coil结构域特征，推测其参与泛素-蛋白酶体通路的功能机制。
4. **"TRIM73 modulates antiviral immunity by regulating IFN-β signaling"**
*Authors: Li, X. et al.*
摘要：实验表明TRIM73通过K63泛素化修饰MAVS蛋白，增强I型干扰素信号，从而抑制RNA病毒复制。
**注意**：以上为模拟示例，TRIM73相关研究在公开数据库中较为少见，建议进一步检索PubMed、Google Scholar等平台或关注TRIM家族综述文献中的潜在线索。
TRIM73 (Tripartite Motif-containing 73) is a member of the TRIM protein family, characterized by a conserved N-terminal tripartite motif (RING, B-box, and coiled-coil domains). These proteins are involved in diverse cellular processes, including ubiquitination, transcriptional regulation, and antiviral responses. TRIM73. specifically, is encoded by a primate-specific gene located on chromosome 7q11.23. While its exact biological functions remain less characterized compared to other TRIM members, emerging studies suggest roles in germ cell development and tumorigenesis. For instance, TRIM73 is preferentially expressed in the testis and ovaries, hinting at potential involvement in reproductive biology. Structurally, its RING domain may confer E3 ubiquitin ligase activity, allowing participation in protein degradation pathways, though experimental validation is limited. Recent evidence also links TRIM73 dysregulation to cancers, such as hepatocellular carcinoma, where it may act as a tumor suppressor by modulating apoptosis-related pathways. However, its mechanisms and substrate specificity are poorly understood. Recombinant TRIM73 protein, produced via heterologous expression systems, serves as a tool for studying its interactions, enzymatic properties, and post-translational modifications. Current research remains focused on elucidating its physiological relevance, disease associations, and potential as a therapeutic target or biomarker. Further investigations are needed to unravel its functional diversity within the TRIM family and its broader implications in human health.
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