| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM6 |
| Uniprot No | Q9C030 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-488 aa |
| 活性数据 | MTSPVLVDIR EEVTCPICLE LLTEPLSIDC GHSFCQACIT PNGRESVIGQ EGERSCPVCQ TSYQPGNLRP NRHLANIVRR LREVVLGPGK QLKAVLCADH GEKLQLFCQE DGKVICWLCE RSQEHRGHHT FLVEEVAQEY QEKFQESLKK LKNEEQEAEK LTAFIREKKT SWKNQMEPER CRIQTEFNQL RNILDRVEQR ELKKLEQEEK KGLRIIEEAE NDLVHQTQSL RELISDLERR CQGSTMELLQ DVSDVTERSE FWTLRKPEAL PTKLRSMFRA PDLKRMLRVC RELTDVQSYW VDVTLNPHTA NLNLVLAKNR RQVRFVGAKV SGPSCLEKHY DCSVLGSQHF SSGKHYWEVD VAKKTAWILG VCSNSLGPTF SFNHFAQNHS AYSRYQPQSG YWVIGLQHNH EYRAYEDSSP SLLLSMTVPP RRVGVFLDYE AGTVSFYNVT NHGFPIYTFS KYYFPTTLCP YFNPCNCVIP MTLRRPSS |
| 分子量 | 56.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRIM6蛋白的3篇参考文献的简要概括：
1. **文献名称**: *TRIM6 interacts with the mitochondrial E3 ubiquitin ligase ARHH to activate antiviral innate immunity*
**作者**: Zhang Y. et al.
**摘要**: 研究揭示TRIM6通过与线粒体E3泛素连接酶ARHH相互作用，促进抗病毒天然免疫反应，增强I型干扰素的产生，抑制RNA病毒复制。
2. **文献名称**: *TRIM6 directs the IKKε-mediated antiviral response to unanchored ubiquitin chains*
**作者**: Rajsbaum R. et al.
**摘要**: TRIM6被证实通过催化非锚定K48泛素链的合成，激活IKKε激酶，进而促进干扰素调节因子3(IRF3)的磷酸化，增强宿主对病毒感染的免疫应答。
3. **文献名称**: *TRIM6 is required for K63-linked ubiquitination and NF-κB activation in cancer metastasis*
**作者**: Liu B. et al.
**摘要**: 研究表明TRIM6通过介导K63泛素链的形成，激活NF-κB信号通路，促进肿瘤细胞的迁移和侵袭，揭示其在癌症转移中的关键作用。
如需具体文献来源，可进一步提供DOI或PMID进行检索。
TRIM6 (Tripartite Motif-Containing Protein 6) is a member of the TRIM family of proteins, characterized by a conserved N-terminal tripartite motif comprising a RING finger domain, one or two B-box domains, and a coiled-coil region. These modular domains enable TRIM proteins to function as E3 ubiquitin ligases, mediating post-translational protein modifications through ubiquitination. TRIM6 is primarily localized in the cytoplasm and has been implicated in diverse cellular processes, including innate immunity, antiviral defense, and cellular stress responses.
A key function of TRIM6 involves its role in the type I interferon (IFN) signaling pathway, where it facilitates the synthesis of unanchored K48-linked ubiquitin chains. These chains act as signaling platforms to activate IKKε (IKBKE), a kinase critical for phosphorylating interferon regulatory factors (IRFs) and initiating antiviral gene expression. Studies highlight TRIM6's importance in restricting RNA viruses, such as influenza A and HIV-1. by promoting interferon-stimulated gene (ISG) production. Additionally, TRIM6 interacts with the ubiquitin-conjugating enzyme UE2K (UBE2K) to regulate substrate-specific ubiquitination, influencing processes like protein quality control and DNA damage repair.
Emerging evidence links TRIM6 to cancer progression and inflammatory diseases, with dysregulated expression observed in certain malignancies. Its dual role in promoting tumor cell survival or death, depending on cellular context, underscores its complexity. While much remains unknown about its precise mechanisms, TRIM6 represents a promising therapeutic target for antiviral and anticancer strategies. Ongoing research focuses on elucidating its substrate specificity, regulation of innate immune pathways, and crosstalk with other TRIM proteins.
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