| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM65 |
| Uniprot No | Q6PJ69 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-517 aa |
| 活性数据 | AAQLLEEKLTCAICLGLYQDPVTLPCGHNFCGACIRDWWDRCGKACPECREPFPDGAELRRNVALSGVLEVVRAGPARDPGPDPGPGPDPAARCPRHGRPLELFCRTEGRCVCSVCTVRECRLHERALLDAERLKREAQLRASLEVTQQQATQAEGQLLELRKQSSQIQNSACILASWVSGKFSSLLQALEIQHTTALRSIEVAKTQALAQARDEEQRLRVHLEAVARHGCRIRELLEQVDEQTFLQESQLLQPPGPLGPLTPLQWDEDQQLGDLKQLLSRLCGLLLEEGSHPGAPAKPVDLAPVEAPGPLAPVPSTVCPLRRKLWQNYRNLTFDPVSANRHFYLSRQDQQVKHCRQSRGPGGPGSFELWQVQCAQSFQAGHHYWEVRASDHSVTLGVSYPQLPRCRLGPHTDNIGRGPCSWGLCVQEDSLQAWHNGEAQRLPGVSGRLLGMDLDLASGCLTFYSLEPQTQPLYTFHALFNQPLTPVFWLLEGRTLTLCHQPGAVFPLGPQEEVLS |
| 分子量 | 64.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"TRIM65 regulates antiviral immune response through ubiquitination of MDA5"**
- **作者**: Wang et al. (2018)
- **摘要**: 研究发现TRIM65作为E3泛素连接酶，通过促进MDA5的K63泛素化增强抗RNA病毒(如VSV)的先天免疫反应，揭示其在RIG-I样受体信号通路中的关键作用。
2. **"TRIM65 promotes non-small cell lung cancer progression by modulating Wnt/β-catenin signaling"**
- **作者**: Liu et al. (2020)
- **摘要**: 本文证实TRIM65在非小细胞肺癌(NSCLC)中高表达，通过稳定β-catenin蛋白激活Wnt通路，促进肿瘤细胞增殖和侵袭，提示其作为潜在治疗靶点。
3. **"TRIM65 suppresses hepatitis B virus replication by targeting HBx for ubiquitination and degradation"**
- **作者**: Chen et al. (2019)
- **摘要**: 研究显示TRIM65通过泛素化降解乙型肝炎病毒(HBV)的X蛋白(HBx)，抑制病毒复制，为抗HBV机制提供了新视角。
4. **"TRIM65-dependent degradation of AGO2 inhibits cancer cell proliferation"**
- **作者**: Li et al. (2016)
- **摘要**: TRIM65通过介导AGO2的泛素化降解，抑制microRNA介导的基因沉默，在结直肠癌中发挥抑癌作用，揭示其与肿瘤进展的关联。
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**注**：以上为模拟简化版文献，实际引用需通过PubMed/Google Scholar等平台检索具体文章。
TRIM65. a member of the tripartite motif (TRIM) protein family, is characterized by its conserved RING finger, B-box, and coiled-coil domains. This ubiquitin E3 ligase plays multifaceted roles in cellular processes, including protein degradation, innate immune signaling, and antiviral defense. It regulates substrate ubiquitination, influencing proteasomal or autophagic pathways, and interacts with key immune regulators like MAVS to modulate interferon production during viral infections.
In cancer biology, TRIM65 exhibits context-dependent duality, acting as an oncogene or tumor suppressor. It promotes tumor progression in lung and colorectal cancers by destabilizing tumor suppressors (e.g., TIAR) or enhancing oncogenic pathways, while suppressing hepatocellular carcinoma via PTEN/AKT axis regulation. Its involvement extends to neuropathologies, with altered expression observed in Alzheimer’s disease and prion disorders, potentially affecting neuroinflammation and protein aggregation.
Emerging studies highlight TRIM65's regulatory effects on RNA metabolism, including microRNA processing and mRNA stability, through interactions with RNA-binding proteins. Despite progress, its tissue-specific functions, substrate network, and therapeutic potential remain under exploration. Current research focuses on deciphering its mechanistic complexity across diseases and evaluating targeting strategies for cancer and neurodegenerative conditions.
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